The interaction of carbon-14-labelled alkyl carbamates, labelled in the alkyl and carbonyl positions, with DNA in vivo

Terence A. Lawson, Allan W. Pound

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Abstract

The binding of ethyl carbamate labelled with carbon-14 in the alkyl or carbonyl group, and of methyl, n-butyl and n-propyl carbamates labelled in the alkyl group, to the DNA of mouse liver, lung and kidney has been studied in male Crackenbush mice. Only ethyl carbamate bound to liver and kidney DNA to any significant extent. The binding of ethyl carbamate labelled with carbon-14 in the C1, C2 or the carbonyl position was examined and compared. The levels of binding of [1-14C]- and [2-14C]ethyl carbamate to liver DNA were not significantly different (328 ± 34 and 267 ± 24 dpm/mg DNA, respectively), but there was very little binding of the [carbonyl-14C]ethyl carbamate (26 ± 3 dpm/mg DNA). Furthermore, only 18% of the radioactivity was removed from the DNA labelled with the alkyl-labelled carbamates, whereas 65% of the radioactivity was removed from the DNA labelled with carbonyl-labelled ethyl carbamate on continuous ether extraction. It was concluded that the bound molecule does not contain the carbonyl carbon and is probably an ethyl group.

Original languageEnglish (US)
Pages (from-to)99-105
Number of pages7
JournalChemico-Biological Interactions
Volume6
Issue number2
DOIs
Publication statusPublished - Feb 1973

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ASJC Scopus subject areas

  • Toxicology

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