The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3

Barbara L. Gaffney, Luis A. Marky, Roger A. Jones

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A self-complementary hexanucleotide consisting of thymidine and 2-aminodeoxyadenosine, d(TA'), has been synthesized by a solid phase phosphotriester method. Melting studies show that the additional hydrogen bond afforded by the 2-amino group substantially stabilizes the duplex. Moreover, conformational analysis using circulardichroim shows that a salt-induced conformational transition occurs, similar to the B→Z transition observed for d(CG)n oligonucleotides.

Original languageEnglish (US)
Pages (from-to)4351-4361
Number of pages11
JournalNucleic acids research
Volume10
Issue number14
DOIs
StatePublished - Jul 24 1982

Fingerprint

Nucleic Acid Conformation
Oligonucleotides
Conformation
Thymidine
Freezing
Conformations
Hydrogen
Hydrogen bonds
Melting
DNA
Salts
Synthesis
Hydrogen Bonds
Salt
deoxythymidylyl-3'-5'-deoxyadenylate
purine
Influence

ASJC Scopus subject areas

  • Genetics

Cite this

The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3. / Gaffney, Barbara L.; Marky, Luis A.; Jones, Roger A.

In: Nucleic acids research, Vol. 10, No. 14, 24.07.1982, p. 4351-4361.

Research output: Contribution to journalArticle

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