The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3

Barbara L. Gaffney, Luis A. Marky, Roger A. Jones

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A self-complementary hexanucleotide consisting of thymidine and 2-aminodeoxyadenosine, d(TA'), has been synthesized by a solid phase phosphotriester method. Melting studies show that the additional hydrogen bond afforded by the 2-amino group substantially stabilizes the duplex. Moreover, conformational analysis using circulardichroim shows that a salt-induced conformational transition occurs, similar to the B→Z transition observed for d(CG)n oligonucleotides.

Original languageEnglish (US)
Pages (from-to)4351-4361
Number of pages11
JournalNucleic acids research
Issue number14
Publication statusPublished - Jul 24 1982


ASJC Scopus subject areas

  • Genetics

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