The Ig-Like Domain of Tapasin Influences Intermolecular Interactions

Heth R. Turnquist, Jason L. Petersen, Shanna E. Vargas, Mary M. McIlhaney, Elliott Bedows, Werner E. Mayer, Andres G. Grandea, Luc Van Kaer, Joyce C Solheim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Presentation of antigenic peptides to T lymphocytes by MHC class I molecules is regulated by events involving multiple endoplasmic reticulum proteins, including tapasin. By studying the effects of substitutions in the tapasin Ig-like domain, we demonstrated that H-2Ld/tapasin association can be segregated from reconstitution of folded Ld surface expression. This finding suggests that peptide acquisition by L d is influenced by tapasin functions that are independent of L d binding. We also found that the presence of a nine-amino acid region in the Ig-like domain of mouse or human tapasin is required for association with Ld, and certain point substitutions in this sequence abrogate human, but not mouse, tapasin association with Ld. These data are consistent with a higher overall affinity between Ld and mouse tapasin compared with human tapasin. In addition, we found that other point mutations in the same region of the tapasin Ig-like domain affect MHC class I surface expression and Ag presentation. Finally, we showed that the cysteine residues in the Ig-like domain of tapasin influence tapasin's stability, its interaction with the MHC class I H chain, and its stabilization of TAP. Mutagenesis of these cysteines decreases tapasin's electrophoretic mobility, suggesting that these residues form an intramolecular disuffide bond. Taken together, these results reveal a critical role for the tapasin Ig-like domain in tapasin function.

Original languageEnglish (US)
Pages (from-to)2976-2984
Number of pages9
JournalJournal of Immunology
Volume172
Issue number5
DOIs
StatePublished - Mar 1 2004

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tapasin
Immunoglobulin Domains
Cysteine
Peptide T
Point Mutation
Mutagenesis
Endoplasmic Reticulum
T-Lymphocytes
Amino Acids
Proteins
peptide L

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Turnquist, H. R., Petersen, J. L., Vargas, S. E., McIlhaney, M. M., Bedows, E., Mayer, W. E., ... Solheim, J. C. (2004). The Ig-Like Domain of Tapasin Influences Intermolecular Interactions. Journal of Immunology, 172(5), 2976-2984. https://doi.org/10.4049/jimmunol.172.5.2976

The Ig-Like Domain of Tapasin Influences Intermolecular Interactions. / Turnquist, Heth R.; Petersen, Jason L.; Vargas, Shanna E.; McIlhaney, Mary M.; Bedows, Elliott; Mayer, Werner E.; Grandea, Andres G.; Van Kaer, Luc; Solheim, Joyce C.

In: Journal of Immunology, Vol. 172, No. 5, 01.03.2004, p. 2976-2984.

Research output: Contribution to journalArticle

Turnquist, HR, Petersen, JL, Vargas, SE, McIlhaney, MM, Bedows, E, Mayer, WE, Grandea, AG, Van Kaer, L & Solheim, JC 2004, 'The Ig-Like Domain of Tapasin Influences Intermolecular Interactions', Journal of Immunology, vol. 172, no. 5, pp. 2976-2984. https://doi.org/10.4049/jimmunol.172.5.2976
Turnquist HR, Petersen JL, Vargas SE, McIlhaney MM, Bedows E, Mayer WE et al. The Ig-Like Domain of Tapasin Influences Intermolecular Interactions. Journal of Immunology. 2004 Mar 1;172(5):2976-2984. https://doi.org/10.4049/jimmunol.172.5.2976
Turnquist, Heth R. ; Petersen, Jason L. ; Vargas, Shanna E. ; McIlhaney, Mary M. ; Bedows, Elliott ; Mayer, Werner E. ; Grandea, Andres G. ; Van Kaer, Luc ; Solheim, Joyce C. / The Ig-Like Domain of Tapasin Influences Intermolecular Interactions. In: Journal of Immunology. 2004 ; Vol. 172, No. 5. pp. 2976-2984.
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