The Identity of Proteins Associated with a Small Heat Shock Potein during Heat Stress in Vivo Indicates That These Chaperones Protect a Wide Range of Cellular Functions

Eman Basha, Garrett J. Lee, Linda A. Breci, Andrew C. Hausrath, Nicole R. Buan, Kim C. Giese, Elizabeth Vierling

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

The small heat shock proteins (sHSPs) are a ubiquitous class of ATP-independent chaperones believed to prevent irreversible protein aggregation and to facilitate subsequent protein renaturation in cooperation with ATP-dependent chaperones. Although sHSP chaperone activity has been studied extensively in vitro, understanding the mechanism of sHSP function requires identification of proteins that are sHSP substrates in vivo. We have used both immunoprecipitation and affinity chromatography to recover 42 proteins that specifically interact with Synechocystis Hsp16.6 in vivo during heat treatment. These proteins can all be released from Hsp16.6 by the ATP-dependent activity of DnaK and cochaperones and are heat-labile. Thirteen of the putative substrate proteins were identified by mass spectrometry and reveal the potential for sHSPs to protect cellular functions as diverse as transcription, translation, cell signaling, and secondary metabolism. One of the putative substrates, serine esterase, was purified and tested directly for interaction with purified Hsp16.6. Hsp16.6 effectively formed soluble complexes with serine esterase in a heat-dependent fashion, thereby preventing formation of insoluble serine esterase aggregates. These data offer critical insights into the characteristics of native sHSP substrates and extend and provide in vivo support for the chaperone model of sHSP function.

Original languageEnglish (US)
Pages (from-to)7566-7575
Number of pages10
JournalJournal of Biological Chemistry
Volume279
Issue number9
DOIs
StatePublished - Feb 27 2004

Fingerprint

Small Heat-Shock Proteins
Shock
Hot Temperature
Proteins
Adenosine Triphosphate
Substrates
Protein Renaturation
Secondary Metabolism
Cell signaling
Synechocystis
Affinity chromatography
Transcription
Affinity Chromatography
Immunoprecipitation
Mass spectrometry
Mass Spectrometry
Agglomeration
Heat treatment

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The Identity of Proteins Associated with a Small Heat Shock Potein during Heat Stress in Vivo Indicates That These Chaperones Protect a Wide Range of Cellular Functions. / Basha, Eman; Lee, Garrett J.; Breci, Linda A.; Hausrath, Andrew C.; Buan, Nicole R.; Giese, Kim C.; Vierling, Elizabeth.

In: Journal of Biological Chemistry, Vol. 279, No. 9, 27.02.2004, p. 7566-7575.

Research output: Contribution to journalArticle

Basha, Eman ; Lee, Garrett J. ; Breci, Linda A. ; Hausrath, Andrew C. ; Buan, Nicole R. ; Giese, Kim C. ; Vierling, Elizabeth. / The Identity of Proteins Associated with a Small Heat Shock Potein during Heat Stress in Vivo Indicates That These Chaperones Protect a Wide Range of Cellular Functions. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 9. pp. 7566-7575.
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