The human PAF complex coordinates transcription with events downstream of RNA synthesis

Bing Zhu, Subhrangsu S. Mandal, Anh Dung Pham, Yong Zheng, Hediye Erdjument-Bromage, Surinder Kumar Batra, Paul Tempst, Danny Reinberg

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

The yeast PAF (yPAF) complex interacts with RNA polymerase II and coordinates the setting of histone marks associated with active transcription. We report the isolation and functional characterization of the human PAF (hPAF) complex. hPAF shares four subunits with yPAF (hCtr9, hPaf1, hLeo1, and hCdc73), but contains a novel higher eukaryotic-specific subunit, hSki8. RNAi against hSki8 or hCtr9 reduces the cellular levels of other hPAF subunits and of mono- and trimethylated H3-Lys 4 and dimethylated H3-Lys 79. The hSki8 subunit is also a component of the human SKI (hSKI) complex. Yeast SKI complex is cytoplasmic and together with Exosome mediates 3′-5′ mRNA degradation. However, hSKI complex localizes to both nucleus and cytoplasm. Immunoprecipitation experiments revealed that hPAF and hSKI complexes interact, and ChIP experiments demonstrated that hSKI associates with transcriptionally active genes dependent on the presence of hPAF. Thus, in addition to coordinating events during transcription (initiation, promoter clearance, and elongation), hPAF also coordinates events in RNA quality control.

Original languageEnglish (US)
Pages (from-to)1668-1673
Number of pages6
JournalGenes and Development
Volume19
Issue number14
DOIs
StatePublished - Jul 1 2005

Fingerprint

RNA
Yeasts
Histone Code
Exosomes
RNA Polymerase II
RNA Stability
RNA Interference
Immunoprecipitation
Quality Control
Cytoplasm
Genes

Keywords

  • PAF
  • RNA surveillance
  • SKI
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Zhu, B., Mandal, S. S., Pham, A. D., Zheng, Y., Erdjument-Bromage, H., Batra, S. K., ... Reinberg, D. (2005). The human PAF complex coordinates transcription with events downstream of RNA synthesis. Genes and Development, 19(14), 1668-1673. https://doi.org/10.1101/gad.1292105

The human PAF complex coordinates transcription with events downstream of RNA synthesis. / Zhu, Bing; Mandal, Subhrangsu S.; Pham, Anh Dung; Zheng, Yong; Erdjument-Bromage, Hediye; Batra, Surinder Kumar; Tempst, Paul; Reinberg, Danny.

In: Genes and Development, Vol. 19, No. 14, 01.07.2005, p. 1668-1673.

Research output: Contribution to journalArticle

Zhu, B, Mandal, SS, Pham, AD, Zheng, Y, Erdjument-Bromage, H, Batra, SK, Tempst, P & Reinberg, D 2005, 'The human PAF complex coordinates transcription with events downstream of RNA synthesis', Genes and Development, vol. 19, no. 14, pp. 1668-1673. https://doi.org/10.1101/gad.1292105
Zhu, Bing ; Mandal, Subhrangsu S. ; Pham, Anh Dung ; Zheng, Yong ; Erdjument-Bromage, Hediye ; Batra, Surinder Kumar ; Tempst, Paul ; Reinberg, Danny. / The human PAF complex coordinates transcription with events downstream of RNA synthesis. In: Genes and Development. 2005 ; Vol. 19, No. 14. pp. 1668-1673.
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