The Human Immunodeficiency Virus Long Terminal Repeat Is Preferentially Expressed in Langerhans Cells in Transgenic Mice

John Leonard, Jaspal S. Khillan, Howard E. Gendelman, Akio Adachi, Shaun Lorenzo, Heiner Westphal, Malcolm A. Martin, Monte S. Meltzer

Research output: Contribution to journalArticle

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Abstract

Four lines of transgenic mice containing the HIV LTR linked to the bacterial gene encoding chloramphenicol acetyltransferase (CAT) were constructed. In each line, a characteristic tissue pattern of CAT expression was observed with detectable levels present in the eye, heart, spleen, thymus, and tail. Low levels of CAT were present in circulating lymphocytes, but CAT activity in these cells could be augmented following treatment with the mitogen phytohemagglutinin (PHA). Likewise, CAT expression was present at only low levels in circulating monocytes, but higher levels of CAT were observed in macrophages grown in the presence of various cytokines (CSF-1, GM-CSF, IL-1α, IL-4, and IL-2). Furthermore, Langerhans cells recovered from skin showed higher levels of CAT activity than those observed in other cells of monocyte-macrophage lineage. These results indicate that LTR-CAT expression in cells of monocyte-macrophage lineage may increase in proportion to the degree of differentiation of these cells. These animals may be useful in the study of cell-specific determinants of LTR-directed gene activity and may serve to identify exogenous cofactors that promote the progression of HIV-related disease in vivo.

Original languageEnglish (US)
Pages (from-to)421-430
Number of pages10
JournalAIDS Research and Human Retroviruses
Volume5
Issue number4
DOIs
StatePublished - Aug 1989

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HIV Long Terminal Repeat
Chloramphenicol O-Acetyltransferase
Langerhans Cells
Transgenic Mice
Monocytes
Macrophage Colony-Stimulating Factor
Macrophages
Granulocyte-Macrophage Colony-Stimulating Factor
HIV
Bacterial Genes
Phytohemagglutinins
Interleukin-1
Mitogens
Interleukin-4
Thymus Gland
Interleukin-2
Tail
Cell Differentiation
Spleen
Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

The Human Immunodeficiency Virus Long Terminal Repeat Is Preferentially Expressed in Langerhans Cells in Transgenic Mice. / Leonard, John; Khillan, Jaspal S.; Gendelman, Howard E.; Adachi, Akio; Lorenzo, Shaun; Westphal, Heiner; Martin, Malcolm A.; Meltzer, Monte S.

In: AIDS Research and Human Retroviruses, Vol. 5, No. 4, 08.1989, p. 421-430.

Research output: Contribution to journalArticle

Leonard, John ; Khillan, Jaspal S. ; Gendelman, Howard E. ; Adachi, Akio ; Lorenzo, Shaun ; Westphal, Heiner ; Martin, Malcolm A. ; Meltzer, Monte S. / The Human Immunodeficiency Virus Long Terminal Repeat Is Preferentially Expressed in Langerhans Cells in Transgenic Mice. In: AIDS Research and Human Retroviruses. 1989 ; Vol. 5, No. 4. pp. 421-430.
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