The H2O2 stimulon in Saccharomyces cerevisiae

Christian Godon, Gilles Lagniel, Jaekwon Lee, Jean Marie Buhler, Sylvie Kieffer, Michel Perrot, Hélian Boucherie, Michel B. Toledano, Jean Labarre

Research output: Contribution to journalArticle

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Abstract

The changes in gene expression underlying the yeast adaptive stress response to H2O2 were analyzed by comparative two-dimensional gel electrophoresis of total cell proteins. The synthesis of at least 115 proteins is stimulated by H2O2, whereas 52 other proteins are repressed by this treatment. We have identified 71 of the stimulated and 44 of the repressed targets. The kinetics and dose-response parameters of the H2O2 genomic response were also analyzed. Identification of these proteins and their mapping into specific cellular processes give a distinct picture of the way in which yeast cells adapt to oxidative stress. As expected, H2O2- responsive targets include an important number of heat shock proteins and proteins with reactive oxygen intermediate scavenging activities. Exposure to H2O2 also results in a slowdown of protein biosynthetic processes and a stimulation of protein degradation pathways. Finally, the most remarkable result inferred from this study is the resetting of carbohydrate metabolism minutes after the exposure to H2O2. Carbohydrate fluxes are redirected to the regeneration of NADPH at the expense of glycolysis. This study represents the first genome-wide characterization of a H2O2-inducible stimulon in a eukaryote.

Original languageEnglish (US)
Pages (from-to)22480-22489
Number of pages10
JournalJournal of Biological Chemistry
Volume273
Issue number35
DOIs
StatePublished - Aug 28 1998

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Yeast
Saccharomyces cerevisiae
Proteins
Yeasts
Electrophoresis, Gel, Two-Dimensional
Carbohydrate Metabolism
Glycolysis
Heat-Shock Proteins
Eukaryota
Oxidative stress
NADP
Recombinant Proteins
Scavenging
Proteolysis
Regeneration
Electrophoresis
Gene expression
Oxidative Stress
Carbohydrates
Genome

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Godon, C., Lagniel, G., Lee, J., Buhler, J. M., Kieffer, S., Perrot, M., ... Labarre, J. (1998). The H2O2 stimulon in Saccharomyces cerevisiae. Journal of Biological Chemistry, 273(35), 22480-22489. https://doi.org/10.1074/jbc.273.35.22480

The H2O2 stimulon in Saccharomyces cerevisiae. / Godon, Christian; Lagniel, Gilles; Lee, Jaekwon; Buhler, Jean Marie; Kieffer, Sylvie; Perrot, Michel; Boucherie, Hélian; Toledano, Michel B.; Labarre, Jean.

In: Journal of Biological Chemistry, Vol. 273, No. 35, 28.08.1998, p. 22480-22489.

Research output: Contribution to journalArticle

Godon, C, Lagniel, G, Lee, J, Buhler, JM, Kieffer, S, Perrot, M, Boucherie, H, Toledano, MB & Labarre, J 1998, 'The H2O2 stimulon in Saccharomyces cerevisiae', Journal of Biological Chemistry, vol. 273, no. 35, pp. 22480-22489. https://doi.org/10.1074/jbc.273.35.22480
Godon C, Lagniel G, Lee J, Buhler JM, Kieffer S, Perrot M et al. The H2O2 stimulon in Saccharomyces cerevisiae. Journal of Biological Chemistry. 1998 Aug 28;273(35):22480-22489. https://doi.org/10.1074/jbc.273.35.22480
Godon, Christian ; Lagniel, Gilles ; Lee, Jaekwon ; Buhler, Jean Marie ; Kieffer, Sylvie ; Perrot, Michel ; Boucherie, Hélian ; Toledano, Michel B. ; Labarre, Jean. / The H2O2 stimulon in Saccharomyces cerevisiae. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 35. pp. 22480-22489.
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