The HopPtoF Locus of Pseudomonas syringae pv. tomato DC3000 encodes a type III chaperone and a cognate effector

Libo Shan, Hye Sook Oh, Jianfu Chen, Ming Guo, Jianmin Zhou, James R. Alfano, Alan Collmer, Xu Jia, Xiaoyan Tang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Type III secretion systems are highly conserved among gram-negative plant and animal pathogenic bacteria. Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells. Analysis of the whole genome sequence of Pseudomonas syringae pv. tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in P. syringae pv. phaseolicola. The HopPtoF locus harbors two genes, ShcF Pto and HopFPto, that are preceded by a single hrp box promoter. We present evidence here to show that ShcFPto and HopF Pto encode a type III chaperone and a cognate effector, respectively. ShcFPto interacts with and stabilizes the HopFPto protein in the bacterial cell. Translation of HopFPto starts at a rare initiation codon ATA that limits the synthesis of the HopFPto protein to a low level in bacterial cells.

Original languageEnglish (US)
Pages (from-to)447-455
Number of pages9
JournalMolecular Plant-Microbe Interactions
Volume17
Issue number5
DOIs
StatePublished - May 2004

Fingerprint

Pseudomonas syringae
Pseudomonas syringae pv. tomato
Lycopersicon esculentum
type III secretion system
Genes
loci
animal pathogenic bacteria
Bacteria
Pseudomonas syringae pv. phaseolicola
plant pathogenic bacteria
start codon
bacterial proteins
Proteins
Bacterial Proteins
Initiator Codon
cells
Ports and harbors
Gram-negative bacteria
translation (genetics)
Virulence

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

The HopPtoF Locus of Pseudomonas syringae pv. tomato DC3000 encodes a type III chaperone and a cognate effector. / Shan, Libo; Oh, Hye Sook; Chen, Jianfu; Guo, Ming; Zhou, Jianmin; Alfano, James R.; Collmer, Alan; Jia, Xu; Tang, Xiaoyan.

In: Molecular Plant-Microbe Interactions, Vol. 17, No. 5, 05.2004, p. 447-455.

Research output: Contribution to journalArticle

Shan, Libo ; Oh, Hye Sook ; Chen, Jianfu ; Guo, Ming ; Zhou, Jianmin ; Alfano, James R. ; Collmer, Alan ; Jia, Xu ; Tang, Xiaoyan. / The HopPtoF Locus of Pseudomonas syringae pv. tomato DC3000 encodes a type III chaperone and a cognate effector. In: Molecular Plant-Microbe Interactions. 2004 ; Vol. 17, No. 5. pp. 447-455.
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AU - Oh, Hye Sook

AU - Chen, Jianfu

AU - Guo, Ming

AU - Zhou, Jianmin

AU - Alfano, James R.

AU - Collmer, Alan

AU - Jia, Xu

AU - Tang, Xiaoyan

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