The High-Resolution, Three-Dimensional Solution Structure of Human Interleukin-4 Determined by Multidimensional Heteronuclear Magnetic Resonance Spectroscopy

Robert Powers, Daniel S. Garrett, Angela M. Gronenborn, G. Marius Clore, Carl J. March, Eric A. Frieden

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

The high-resolution three-dimensional solution structure of recombinant human interleukin-4 (IL-4), a protein of ∼ 15 kDa which plays a key role in the regulation of B and T lymphocytes, has been determined using three- and four-dimensional heteronuclear NMR spectroscopy. The structure is based on a total of 2973 experimental NMR restraints, comprising 2515 approximate interproton distance restraints, 102 distance restraints for 51 backbone hydrogen bonds, and 356 torsion angle restraints. A total of 30 structures was calculated by means of hybrid distance geometry-simulated annealing, and the atomic rms distribution about the mean coordinate positions for residues 8–129 is 0.44 ± 0.03 Å for the backbone atoms, 0.83 ± 0.03 Å for all atoms, and 0.51 ± 0.04 Å for all atoms excluding disordered side chains. The N-and C-terminal residues (1–7 and 130–133, respectively) appear to be disordered. The structure of IL–4 is dominated by a left-handed four-helix bundle with an unusual topology comprising two overhand connections. The linker elements between the helices are formed by either long loops, small helical turns, or short strands. The latter include a mini antiparallel β-sheet. A best fit superposition of the NMR structure of IL-4 with the 2.25 Å resolution crystal structure [Wlodawer, A., Pavlovsky, A., & Gutschina, A. (1992) FEBS Lett. 309, 59–64] yields a backbone atomic rms difference of 1.37 Å which can be mainly attributed to tighter packing of the helices in the crystal structure. This is indicated by an approximately 20% reduction in the axial separation of three pairs of helices (αA–αC, αA–αD, and αC–αD) in the crystal structure relative to the NMR structure and may reflect the greater flexibility of the molecule in solution which is reduced in the crystal due to intermolecular contacts. Comparison of the NMR structure of IL-4 with the X-ray structures of two other related proteins, granulocyte-macrophage colony stimulating factor [Diedrichs, K., Boone, T., & Karplus, P. A. (1992) Science 254, 1779–1782] and human growth hormone [de Vos, A. M., Ultsch, M., & Kossiakoff, A. A. (1992) Science 255, 306–312], that bind to the same hematopoietic superfamily of cell surface receptors reveals a remarkably similar topological fold, despite the absence of any significant overall sequence identity, and substantial differences in the relative lengths of the helices, the lengths and the nature of the various connecting elements, and the pattern and number of disulfide bridges. Indeed, the Cα atom coordinates of 72 and 79 residues of IL-4 can be superimposed on the Cα coordinates of granulocyte–macrophage colony stimulating factor and human growth hormone with rms differences of ∼ 1.7 and 2.0 Å, respectively.

Original languageEnglish (US)
Pages (from-to)6744-6762
Number of pages19
JournalBiochemistry
Volume32
Issue number26
DOIs
StatePublished - Jan 1 1993

Fingerprint

Magnetic resonance spectroscopy
Interleukin-4
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Human Growth Hormone
Atoms
Crystal structure
Biomolecular Nuclear Magnetic Resonance
Colony-Stimulating Factors
Cell Surface Receptors
Granulocyte-Macrophage Colony-Stimulating Factor
Disulfides
T-cells
Hydrogen
Simulated annealing
B-Lymphocytes
Torsional stress
Nuclear magnetic resonance spectroscopy
X-Rays
T-Lymphocytes

ASJC Scopus subject areas

  • Biochemistry

Cite this

The High-Resolution, Three-Dimensional Solution Structure of Human Interleukin-4 Determined by Multidimensional Heteronuclear Magnetic Resonance Spectroscopy. / Powers, Robert; Garrett, Daniel S.; Gronenborn, Angela M.; Clore, G. Marius; March, Carl J.; Frieden, Eric A.

In: Biochemistry, Vol. 32, No. 26, 01.01.1993, p. 6744-6762.

Research output: Contribution to journalArticle

Powers, Robert ; Garrett, Daniel S. ; Gronenborn, Angela M. ; Clore, G. Marius ; March, Carl J. ; Frieden, Eric A. / The High-Resolution, Three-Dimensional Solution Structure of Human Interleukin-4 Determined by Multidimensional Heteronuclear Magnetic Resonance Spectroscopy. In: Biochemistry. 1993 ; Vol. 32, No. 26. pp. 6744-6762.
@article{7184cb19417e426d82d4a0832762ebe3,
title = "The High-Resolution, Three-Dimensional Solution Structure of Human Interleukin-4 Determined by Multidimensional Heteronuclear Magnetic Resonance Spectroscopy",
abstract = "The high-resolution three-dimensional solution structure of recombinant human interleukin-4 (IL-4), a protein of ∼ 15 kDa which plays a key role in the regulation of B and T lymphocytes, has been determined using three- and four-dimensional heteronuclear NMR spectroscopy. The structure is based on a total of 2973 experimental NMR restraints, comprising 2515 approximate interproton distance restraints, 102 distance restraints for 51 backbone hydrogen bonds, and 356 torsion angle restraints. A total of 30 structures was calculated by means of hybrid distance geometry-simulated annealing, and the atomic rms distribution about the mean coordinate positions for residues 8–129 is 0.44 ± 0.03 {\AA} for the backbone atoms, 0.83 ± 0.03 {\AA} for all atoms, and 0.51 ± 0.04 {\AA} for all atoms excluding disordered side chains. The N-and C-terminal residues (1–7 and 130–133, respectively) appear to be disordered. The structure of IL–4 is dominated by a left-handed four-helix bundle with an unusual topology comprising two overhand connections. The linker elements between the helices are formed by either long loops, small helical turns, or short strands. The latter include a mini antiparallel β-sheet. A best fit superposition of the NMR structure of IL-4 with the 2.25 {\AA} resolution crystal structure [Wlodawer, A., Pavlovsky, A., & Gutschina, A. (1992) FEBS Lett. 309, 59–64] yields a backbone atomic rms difference of 1.37 {\AA} which can be mainly attributed to tighter packing of the helices in the crystal structure. This is indicated by an approximately 20{\%} reduction in the axial separation of three pairs of helices (αA–αC, αA–αD, and αC–αD) in the crystal structure relative to the NMR structure and may reflect the greater flexibility of the molecule in solution which is reduced in the crystal due to intermolecular contacts. Comparison of the NMR structure of IL-4 with the X-ray structures of two other related proteins, granulocyte-macrophage colony stimulating factor [Diedrichs, K., Boone, T., & Karplus, P. A. (1992) Science 254, 1779–1782] and human growth hormone [de Vos, A. M., Ultsch, M., & Kossiakoff, A. A. (1992) Science 255, 306–312], that bind to the same hematopoietic superfamily of cell surface receptors reveals a remarkably similar topological fold, despite the absence of any significant overall sequence identity, and substantial differences in the relative lengths of the helices, the lengths and the nature of the various connecting elements, and the pattern and number of disulfide bridges. Indeed, the Cα atom coordinates of 72 and 79 residues of IL-4 can be superimposed on the Cα coordinates of granulocyte–macrophage colony stimulating factor and human growth hormone with rms differences of ∼ 1.7 and 2.0 {\AA}, respectively.",
author = "Robert Powers and Garrett, {Daniel S.} and Gronenborn, {Angela M.} and Clore, {G. Marius} and March, {Carl J.} and Frieden, {Eric A.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1021/bi00077a030",
language = "English (US)",
volume = "32",
pages = "6744--6762",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "26",

}

TY - JOUR

T1 - The High-Resolution, Three-Dimensional Solution Structure of Human Interleukin-4 Determined by Multidimensional Heteronuclear Magnetic Resonance Spectroscopy

AU - Powers, Robert

AU - Garrett, Daniel S.

AU - Gronenborn, Angela M.

AU - Clore, G. Marius

AU - March, Carl J.

AU - Frieden, Eric A.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - The high-resolution three-dimensional solution structure of recombinant human interleukin-4 (IL-4), a protein of ∼ 15 kDa which plays a key role in the regulation of B and T lymphocytes, has been determined using three- and four-dimensional heteronuclear NMR spectroscopy. The structure is based on a total of 2973 experimental NMR restraints, comprising 2515 approximate interproton distance restraints, 102 distance restraints for 51 backbone hydrogen bonds, and 356 torsion angle restraints. A total of 30 structures was calculated by means of hybrid distance geometry-simulated annealing, and the atomic rms distribution about the mean coordinate positions for residues 8–129 is 0.44 ± 0.03 Å for the backbone atoms, 0.83 ± 0.03 Å for all atoms, and 0.51 ± 0.04 Å for all atoms excluding disordered side chains. The N-and C-terminal residues (1–7 and 130–133, respectively) appear to be disordered. The structure of IL–4 is dominated by a left-handed four-helix bundle with an unusual topology comprising two overhand connections. The linker elements between the helices are formed by either long loops, small helical turns, or short strands. The latter include a mini antiparallel β-sheet. A best fit superposition of the NMR structure of IL-4 with the 2.25 Å resolution crystal structure [Wlodawer, A., Pavlovsky, A., & Gutschina, A. (1992) FEBS Lett. 309, 59–64] yields a backbone atomic rms difference of 1.37 Å which can be mainly attributed to tighter packing of the helices in the crystal structure. This is indicated by an approximately 20% reduction in the axial separation of three pairs of helices (αA–αC, αA–αD, and αC–αD) in the crystal structure relative to the NMR structure and may reflect the greater flexibility of the molecule in solution which is reduced in the crystal due to intermolecular contacts. Comparison of the NMR structure of IL-4 with the X-ray structures of two other related proteins, granulocyte-macrophage colony stimulating factor [Diedrichs, K., Boone, T., & Karplus, P. A. (1992) Science 254, 1779–1782] and human growth hormone [de Vos, A. M., Ultsch, M., & Kossiakoff, A. A. (1992) Science 255, 306–312], that bind to the same hematopoietic superfamily of cell surface receptors reveals a remarkably similar topological fold, despite the absence of any significant overall sequence identity, and substantial differences in the relative lengths of the helices, the lengths and the nature of the various connecting elements, and the pattern and number of disulfide bridges. Indeed, the Cα atom coordinates of 72 and 79 residues of IL-4 can be superimposed on the Cα coordinates of granulocyte–macrophage colony stimulating factor and human growth hormone with rms differences of ∼ 1.7 and 2.0 Å, respectively.

AB - The high-resolution three-dimensional solution structure of recombinant human interleukin-4 (IL-4), a protein of ∼ 15 kDa which plays a key role in the regulation of B and T lymphocytes, has been determined using three- and four-dimensional heteronuclear NMR spectroscopy. The structure is based on a total of 2973 experimental NMR restraints, comprising 2515 approximate interproton distance restraints, 102 distance restraints for 51 backbone hydrogen bonds, and 356 torsion angle restraints. A total of 30 structures was calculated by means of hybrid distance geometry-simulated annealing, and the atomic rms distribution about the mean coordinate positions for residues 8–129 is 0.44 ± 0.03 Å for the backbone atoms, 0.83 ± 0.03 Å for all atoms, and 0.51 ± 0.04 Å for all atoms excluding disordered side chains. The N-and C-terminal residues (1–7 and 130–133, respectively) appear to be disordered. The structure of IL–4 is dominated by a left-handed four-helix bundle with an unusual topology comprising two overhand connections. The linker elements between the helices are formed by either long loops, small helical turns, or short strands. The latter include a mini antiparallel β-sheet. A best fit superposition of the NMR structure of IL-4 with the 2.25 Å resolution crystal structure [Wlodawer, A., Pavlovsky, A., & Gutschina, A. (1992) FEBS Lett. 309, 59–64] yields a backbone atomic rms difference of 1.37 Å which can be mainly attributed to tighter packing of the helices in the crystal structure. This is indicated by an approximately 20% reduction in the axial separation of three pairs of helices (αA–αC, αA–αD, and αC–αD) in the crystal structure relative to the NMR structure and may reflect the greater flexibility of the molecule in solution which is reduced in the crystal due to intermolecular contacts. Comparison of the NMR structure of IL-4 with the X-ray structures of two other related proteins, granulocyte-macrophage colony stimulating factor [Diedrichs, K., Boone, T., & Karplus, P. A. (1992) Science 254, 1779–1782] and human growth hormone [de Vos, A. M., Ultsch, M., & Kossiakoff, A. A. (1992) Science 255, 306–312], that bind to the same hematopoietic superfamily of cell surface receptors reveals a remarkably similar topological fold, despite the absence of any significant overall sequence identity, and substantial differences in the relative lengths of the helices, the lengths and the nature of the various connecting elements, and the pattern and number of disulfide bridges. Indeed, the Cα atom coordinates of 72 and 79 residues of IL-4 can be superimposed on the Cα coordinates of granulocyte–macrophage colony stimulating factor and human growth hormone with rms differences of ∼ 1.7 and 2.0 Å, respectively.

UR - http://www.scopus.com/inward/record.url?scp=0027236528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027236528&partnerID=8YFLogxK

U2 - 10.1021/bi00077a030

DO - 10.1021/bi00077a030

M3 - Article

VL - 32

SP - 6744

EP - 6762

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 26

ER -