The herpesvirus VP1/2 protein is an effector of dynein-mediated capsid transport and neuroinvasion

Sofia V. Zaichick, Kevin P. Bohannon, Ami Hughes, Patricia J Sollars, Gary E Pickard, Gregory A. Smith

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Microtubule transport of herpesvirus capsids from the cell periphery to the nucleus is imperative for viral replication and, in the case of many alphaherpesviruses, transmission into the nervous system. Using the neuroinvasive herpesvirus, pseudorabies virus (PRV), we show that the viral protein 1/2 (VP1/2) tegument protein associates with the dynein/dynactin microtubule motor complex and promotes retrograde microtubule transport of PRV capsids. Functional activation of VP1/2 requires binding to the capsid protein pUL25 or removal of the capsid-binding domain. A proline-rich sequence within VP1/2 is required for the efficient interaction with the dynein/dynactin microtubule motor complex as well as for PRV virulence and retrograde axon transport in vivo. Additionally, in the absence of infection, functionally active VP1/2 is sufficient to move large surrogate cargoes via the dynein/dynactin microtubule motor complex. Thus, VP1/2 tethers PRV capsids to dynein/dynactin to enhance microtubule transport, neuroinvasion, and pathogenesis.

Original languageEnglish (US)
Pages (from-to)193-203
Number of pages11
JournalCell Host and Microbe
Volume13
Issue number2
DOIs
StatePublished - Feb 13 2013

Fingerprint

Dyneins
Capsid
Herpesviridae
Viral Proteins
Microtubules
Suid Herpesvirus 1
Proteins
Capsid Proteins
Proline
Nervous System
Axons
Virulence
Dynactin Complex
Infection

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

Cite this

The herpesvirus VP1/2 protein is an effector of dynein-mediated capsid transport and neuroinvasion. / Zaichick, Sofia V.; Bohannon, Kevin P.; Hughes, Ami; Sollars, Patricia J; Pickard, Gary E; Smith, Gregory A.

In: Cell Host and Microbe, Vol. 13, No. 2, 13.02.2013, p. 193-203.

Research output: Contribution to journalArticle

Zaichick, Sofia V. ; Bohannon, Kevin P. ; Hughes, Ami ; Sollars, Patricia J ; Pickard, Gary E ; Smith, Gregory A. / The herpesvirus VP1/2 protein is an effector of dynein-mediated capsid transport and neuroinvasion. In: Cell Host and Microbe. 2013 ; Vol. 13, No. 2. pp. 193-203.
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