The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) protects cells against cold-shock-induced apoptosis by maintaining phosphorylation of protein kinase B (AKT)

Dale Carpenter, Chinhui Hsiang, Xianzhi Jiang, Nelson Osorio, Lbachir BenMohamed, Clinton Jones, Steven L. Wechsler

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) blocks apoptosis and inhibits caspase-3 activation. We previously showed that serum starvation (removal of serum from tissue culture media), which takes several days to induce apoptosis, results in decreased levels of both AKT (protein kinase B) and phosphorylated AKT (pAKT) in cells not expressing LAT. In contrast in mouse neuroblastoma cells expressing LAT, AKT, and pAKT levels remained high. AKT is a serine/threonine protein kinase that promotes cell survival. To examine the effect of LAT on AKT-pAKT using a different and more rapid method of inducing apoptosis, a stable cell line expressing LAT was compared to non-LAT expressing cells as soon as 15 min following recovery from cold-shock-induced apoptosis. Expression of LAT appeared to inhibit dephosphorylation of pAKT. This protection correlated with blocking numerous pro-apoptotic events that are inhibited by pAKT. These results support the hypothesis that inhibiting dephosphorylation of pAKT may be one of the pathways by which LAT protects cells against apoptosis.

Original languageEnglish (US)
Pages (from-to)568-575
Number of pages8
JournalJournal of neurovirology
Volume21
Issue number5
DOIs
StatePublished - Oct 1 2015

Fingerprint

Proto-Oncogene Proteins c-akt
Human Herpesvirus 1
Shock
Phosphorylation
Apoptosis
Protein-Serine-Threonine Kinases
Starvation
Serum
Neuroblastoma
Caspase 3
Culture Media
Cell Survival
herpes simplex virus-1 latency associated transcript
Cell Line

Keywords

  • AKT
  • Cold-shock-induced apoptosis
  • Herpes simplex virus type 1
  • Latency-associated transcript

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Virology

Cite this

The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) protects cells against cold-shock-induced apoptosis by maintaining phosphorylation of protein kinase B (AKT). / Carpenter, Dale; Hsiang, Chinhui; Jiang, Xianzhi; Osorio, Nelson; BenMohamed, Lbachir; Jones, Clinton; Wechsler, Steven L.

In: Journal of neurovirology, Vol. 21, No. 5, 01.10.2015, p. 568-575.

Research output: Contribution to journalArticle

Carpenter, Dale ; Hsiang, Chinhui ; Jiang, Xianzhi ; Osorio, Nelson ; BenMohamed, Lbachir ; Jones, Clinton ; Wechsler, Steven L. / The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) protects cells against cold-shock-induced apoptosis by maintaining phosphorylation of protein kinase B (AKT). In: Journal of neurovirology. 2015 ; Vol. 21, No. 5. pp. 568-575.
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