The HARP domain dictates the annealing helicase activity of HARP/SMARCAL1

Gargi Ghosal, Jingsong Yuan, Junjie Chen

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Mutations in HepA-related protein (HARP, or SMARCAL1) cause Schimke immunoosseous dysplasia (SIOD). HARP has ATP-dependent annealing helicase activity, which helps to stabilize stalled replication forks and facilitate DNA repair during replication. Here, we show that the conserved tandem HARP (2HP) domain dictates this annealing helicase activity. Furthermore, chimeric proteins generated by fusing the 2HP domain of HARP with the SNF2 domain of BRG1 or HELLS show annealing helicase activity in vitro and, when targeted to replication forks, mimic the functions of HARP in vivo. We propose that the HARP domain endows HARP with this ATP-driven annealing helicase activity.

Original languageEnglish (US)
Pages (from-to)574-580
Number of pages7
JournalEMBO Reports
Volume12
Issue number6
DOIs
Publication statusPublished - Jun 1 2011

    Fingerprint

Keywords

  • HARP
  • RPA
  • Schimke immunoosseous dysplasia
  • annealing helicase
  • replication

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this