The Growth of Cells in Serum-Free Hormone-Supplemented Media

Jane Bottenstein, Izumi Hayashi, Sharon Hutchings, Hideo Masui, Jennie Mather, Don B. McClure, Sugayuki Ohasa, A Angie Rizzino, Gordon Sato, Ginette Serrero, Richard Wolfe, Reen Wu

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

This chapter presents the practical details for the growth of cells in serum free hormone supplemented media. In the absence of serum, greater than usual care must be taken in preparation of the synthetic portion of the medium. Careful preparation of water is essential for consistent results with serum-free medium. Serum and even dialyzed serum can mask nutritional requirements of cells in culture. Commercially available powdered media are adequate for serum-free work although each batch must be checked for suitability. Serum also serves the function of a trypsin inhibitor in conventional tissue culture procedures. The serum may be necessary for the repair of trypsinization damage after subculture; or the residual serum left after its removal, even after one or more washes, may be furnishing unknown growth factors.

Original languageEnglish (US)
Pages (from-to)94-109
Number of pages16
JournalMethods in enzymology
Volume58
Issue numberC
DOIs
StatePublished - Jan 1 1979

Fingerprint

Serum-Free Culture Media
Cells
Hormones
Tissue culture
Trypsin Inhibitors
Growth
Serum
Masks
Intercellular Signaling Peptides and Proteins
Repair
Water
Nutritional Requirements
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Bottenstein, J., Hayashi, I., Hutchings, S., Masui, H., Mather, J., McClure, D. B., ... Wu, R. (1979). The Growth of Cells in Serum-Free Hormone-Supplemented Media. Methods in enzymology, 58(C), 94-109. https://doi.org/10.1016/S0076-6879(79)58127-0

The Growth of Cells in Serum-Free Hormone-Supplemented Media. / Bottenstein, Jane; Hayashi, Izumi; Hutchings, Sharon; Masui, Hideo; Mather, Jennie; McClure, Don B.; Ohasa, Sugayuki; Rizzino, A Angie; Sato, Gordon; Serrero, Ginette; Wolfe, Richard; Wu, Reen.

In: Methods in enzymology, Vol. 58, No. C, 01.01.1979, p. 94-109.

Research output: Contribution to journalArticle

Bottenstein, J, Hayashi, I, Hutchings, S, Masui, H, Mather, J, McClure, DB, Ohasa, S, Rizzino, AA, Sato, G, Serrero, G, Wolfe, R & Wu, R 1979, 'The Growth of Cells in Serum-Free Hormone-Supplemented Media', Methods in enzymology, vol. 58, no. C, pp. 94-109. https://doi.org/10.1016/S0076-6879(79)58127-0
Bottenstein J, Hayashi I, Hutchings S, Masui H, Mather J, McClure DB et al. The Growth of Cells in Serum-Free Hormone-Supplemented Media. Methods in enzymology. 1979 Jan 1;58(C):94-109. https://doi.org/10.1016/S0076-6879(79)58127-0
Bottenstein, Jane ; Hayashi, Izumi ; Hutchings, Sharon ; Masui, Hideo ; Mather, Jennie ; McClure, Don B. ; Ohasa, Sugayuki ; Rizzino, A Angie ; Sato, Gordon ; Serrero, Ginette ; Wolfe, Richard ; Wu, Reen. / The Growth of Cells in Serum-Free Hormone-Supplemented Media. In: Methods in enzymology. 1979 ; Vol. 58, No. C. pp. 94-109.
@article{55ef085f64694124b5acfeec78ce9944,
title = "The Growth of Cells in Serum-Free Hormone-Supplemented Media",
abstract = "This chapter presents the practical details for the growth of cells in serum free hormone supplemented media. In the absence of serum, greater than usual care must be taken in preparation of the synthetic portion of the medium. Careful preparation of water is essential for consistent results with serum-free medium. Serum and even dialyzed serum can mask nutritional requirements of cells in culture. Commercially available powdered media are adequate for serum-free work although each batch must be checked for suitability. Serum also serves the function of a trypsin inhibitor in conventional tissue culture procedures. The serum may be necessary for the repair of trypsinization damage after subculture; or the residual serum left after its removal, even after one or more washes, may be furnishing unknown growth factors.",
author = "Jane Bottenstein and Izumi Hayashi and Sharon Hutchings and Hideo Masui and Jennie Mather and McClure, {Don B.} and Sugayuki Ohasa and Rizzino, {A Angie} and Gordon Sato and Ginette Serrero and Richard Wolfe and Reen Wu",
year = "1979",
month = "1",
day = "1",
doi = "10.1016/S0076-6879(79)58127-0",
language = "English (US)",
volume = "58",
pages = "94--109",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",
number = "C",

}

TY - JOUR

T1 - The Growth of Cells in Serum-Free Hormone-Supplemented Media

AU - Bottenstein, Jane

AU - Hayashi, Izumi

AU - Hutchings, Sharon

AU - Masui, Hideo

AU - Mather, Jennie

AU - McClure, Don B.

AU - Ohasa, Sugayuki

AU - Rizzino, A Angie

AU - Sato, Gordon

AU - Serrero, Ginette

AU - Wolfe, Richard

AU - Wu, Reen

PY - 1979/1/1

Y1 - 1979/1/1

N2 - This chapter presents the practical details for the growth of cells in serum free hormone supplemented media. In the absence of serum, greater than usual care must be taken in preparation of the synthetic portion of the medium. Careful preparation of water is essential for consistent results with serum-free medium. Serum and even dialyzed serum can mask nutritional requirements of cells in culture. Commercially available powdered media are adequate for serum-free work although each batch must be checked for suitability. Serum also serves the function of a trypsin inhibitor in conventional tissue culture procedures. The serum may be necessary for the repair of trypsinization damage after subculture; or the residual serum left after its removal, even after one or more washes, may be furnishing unknown growth factors.

AB - This chapter presents the practical details for the growth of cells in serum free hormone supplemented media. In the absence of serum, greater than usual care must be taken in preparation of the synthetic portion of the medium. Careful preparation of water is essential for consistent results with serum-free medium. Serum and even dialyzed serum can mask nutritional requirements of cells in culture. Commercially available powdered media are adequate for serum-free work although each batch must be checked for suitability. Serum also serves the function of a trypsin inhibitor in conventional tissue culture procedures. The serum may be necessary for the repair of trypsinization damage after subculture; or the residual serum left after its removal, even after one or more washes, may be furnishing unknown growth factors.

UR - http://www.scopus.com/inward/record.url?scp=0018320872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018320872&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(79)58127-0

DO - 10.1016/S0076-6879(79)58127-0

M3 - Article

VL - 58

SP - 94

EP - 109

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

IS - C

ER -