The gene for a novel transmembrane protein containing epidermal growth factor and follistatin domains is frequently hypermethylated in human tumor cells

G. Liang, K. D. Robertson, C. Talmadge, Janos Sumegi, P. A. Jones

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

A DNA fragment frequently hypermethylated in tumor cells was isolated using a novel screening strategy termed methylation-sensitive arbitrarily primed PCR. The isolated sequence corresponded to a CpG island at the 5' end of a previously unknown gene, TPEF (transmembrane protein containing epidermal growth factor and follistatin domains). Expression of TPEF was observed using Northern master blot analysis of a variety of normal tissues including colon, bladder, and prostate tissue. TPEF maps to human chromosome 2q33, where frequent loss of heterozygosity is seen in various human tumors, and TPEF was not expressed in most human colon and various other tumor cell lines examined by reverse transcription-PCR. Nine of 11 tumor cell lines were highly methylated in the 5' region and the first exon of the gene that demonstrated features characteristic of a CpG island. However the other two cell lines, which expressed TPEF, were hypomethylated in the 5' end of the gene. The region was also hypermethylated in 11 of 16 primary bladder tumors and in 3 of 4 primary colon tumors when compared with adjacent normal tissue. Our results suggest that potential tumor suppressor genes can be isolated from human tumors by virtue of their altered methylation patterns.

Original languageEnglish (US)
Pages (from-to)4907-4912
Number of pages6
JournalCancer Research
Volume60
Issue number17
StatePublished - Sep 1 2000

Fingerprint

Follistatin
Epidermal Growth Factor
Colon
CpG Islands
Tumor Cell Line
Methylation
Genes
Neoplasms
Proteins
Polymerase Chain Reaction
Loss of Heterozygosity
Human Chromosomes
Tumor Suppressor Genes
Urinary Bladder Neoplasms
Northern Blotting
Reverse Transcription
Prostate
Exons
Urinary Bladder
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The gene for a novel transmembrane protein containing epidermal growth factor and follistatin domains is frequently hypermethylated in human tumor cells. / Liang, G.; Robertson, K. D.; Talmadge, C.; Sumegi, Janos; Jones, P. A.

In: Cancer Research, Vol. 60, No. 17, 01.09.2000, p. 4907-4912.

Research output: Contribution to journalArticle

Liang, G. ; Robertson, K. D. ; Talmadge, C. ; Sumegi, Janos ; Jones, P. A. / The gene for a novel transmembrane protein containing epidermal growth factor and follistatin domains is frequently hypermethylated in human tumor cells. In: Cancer Research. 2000 ; Vol. 60, No. 17. pp. 4907-4912.
@article{a431404cc0694d688fa54322730aff58,
title = "The gene for a novel transmembrane protein containing epidermal growth factor and follistatin domains is frequently hypermethylated in human tumor cells",
abstract = "A DNA fragment frequently hypermethylated in tumor cells was isolated using a novel screening strategy termed methylation-sensitive arbitrarily primed PCR. The isolated sequence corresponded to a CpG island at the 5' end of a previously unknown gene, TPEF (transmembrane protein containing epidermal growth factor and follistatin domains). Expression of TPEF was observed using Northern master blot analysis of a variety of normal tissues including colon, bladder, and prostate tissue. TPEF maps to human chromosome 2q33, where frequent loss of heterozygosity is seen in various human tumors, and TPEF was not expressed in most human colon and various other tumor cell lines examined by reverse transcription-PCR. Nine of 11 tumor cell lines were highly methylated in the 5' region and the first exon of the gene that demonstrated features characteristic of a CpG island. However the other two cell lines, which expressed TPEF, were hypomethylated in the 5' end of the gene. The region was also hypermethylated in 11 of 16 primary bladder tumors and in 3 of 4 primary colon tumors when compared with adjacent normal tissue. Our results suggest that potential tumor suppressor genes can be isolated from human tumors by virtue of their altered methylation patterns.",
author = "G. Liang and Robertson, {K. D.} and C. Talmadge and Janos Sumegi and Jones, {P. A.}",
year = "2000",
month = "9",
day = "1",
language = "English (US)",
volume = "60",
pages = "4907--4912",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "17",

}

TY - JOUR

T1 - The gene for a novel transmembrane protein containing epidermal growth factor and follistatin domains is frequently hypermethylated in human tumor cells

AU - Liang, G.

AU - Robertson, K. D.

AU - Talmadge, C.

AU - Sumegi, Janos

AU - Jones, P. A.

PY - 2000/9/1

Y1 - 2000/9/1

N2 - A DNA fragment frequently hypermethylated in tumor cells was isolated using a novel screening strategy termed methylation-sensitive arbitrarily primed PCR. The isolated sequence corresponded to a CpG island at the 5' end of a previously unknown gene, TPEF (transmembrane protein containing epidermal growth factor and follistatin domains). Expression of TPEF was observed using Northern master blot analysis of a variety of normal tissues including colon, bladder, and prostate tissue. TPEF maps to human chromosome 2q33, where frequent loss of heterozygosity is seen in various human tumors, and TPEF was not expressed in most human colon and various other tumor cell lines examined by reverse transcription-PCR. Nine of 11 tumor cell lines were highly methylated in the 5' region and the first exon of the gene that demonstrated features characteristic of a CpG island. However the other two cell lines, which expressed TPEF, were hypomethylated in the 5' end of the gene. The region was also hypermethylated in 11 of 16 primary bladder tumors and in 3 of 4 primary colon tumors when compared with adjacent normal tissue. Our results suggest that potential tumor suppressor genes can be isolated from human tumors by virtue of their altered methylation patterns.

AB - A DNA fragment frequently hypermethylated in tumor cells was isolated using a novel screening strategy termed methylation-sensitive arbitrarily primed PCR. The isolated sequence corresponded to a CpG island at the 5' end of a previously unknown gene, TPEF (transmembrane protein containing epidermal growth factor and follistatin domains). Expression of TPEF was observed using Northern master blot analysis of a variety of normal tissues including colon, bladder, and prostate tissue. TPEF maps to human chromosome 2q33, where frequent loss of heterozygosity is seen in various human tumors, and TPEF was not expressed in most human colon and various other tumor cell lines examined by reverse transcription-PCR. Nine of 11 tumor cell lines were highly methylated in the 5' region and the first exon of the gene that demonstrated features characteristic of a CpG island. However the other two cell lines, which expressed TPEF, were hypomethylated in the 5' end of the gene. The region was also hypermethylated in 11 of 16 primary bladder tumors and in 3 of 4 primary colon tumors when compared with adjacent normal tissue. Our results suggest that potential tumor suppressor genes can be isolated from human tumors by virtue of their altered methylation patterns.

UR - http://www.scopus.com/inward/record.url?scp=0034282986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034282986&partnerID=8YFLogxK

M3 - Article

VL - 60

SP - 4907

EP - 4912

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 17

ER -