The garlic constituent diallyl trisulfide increases the lifespan of C. elegans via skn-1 activation

Anna A. Powolny, Shivendra V. Singh, Simon Melov, Alan Hubbard, Alfred L. Fisher

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Medicinal benefits of Allium vegetables, such as garlic, have been noted throughout recorded history, including protection against cancer and cardiovascular disease. We now demonstrate that garlic constituent diallyl trisulfide (DATS) increases longevity of Caenorhabditis elegans by affecting the skn-1 pathway. Treatment of worms with 5-10 μM DATS increased worm mean lifespan even when treatment is started during young adulthood. To explore the mechanisms involved in the DATS-mediated increase in longevity, we treated daf-2, daf-16, and eat-2 mutants and found that DATS increased the lifespan of daf-2 and daf-16 mutants, but not the eat-2 mutants. Microarray experiments demonstrated that a number of genes regulated by oxidative stress and the skn-1 transcription factor were also changed by DATS treatment. Consistently, DATS treatment leads to the induction of the skn-1 target gene gst-4, and this induction was dependent on skn-1. We also found that the effects of DATS on worm lifespan depend on skn-1 activity in both in the intestine and ASI neurons. Together our data suggest that DATS is able to increase worm lifespan by enhancing the function of the pro-longevity transcription factor skn-1.

Original languageEnglish (US)
Pages (from-to)441-452
Number of pages12
JournalExperimental Gerontology
Volume46
Issue number6
DOIs
StatePublished - Jun 1 2011

Fingerprint

Garlic
Chemical activation
Transcription Factors
Genes
Allium
Oxidative stress
diallyl trisulfide
Caenorhabditis elegans
Vegetables
Microarrays
Neurons
Intestines
Oxidative Stress
Cardiovascular Diseases
History

Keywords

  • Aging
  • C. elegans
  • Diallyl trisulfide
  • Garlic
  • Gst-4
  • Microarray
  • Skn-1

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

The garlic constituent diallyl trisulfide increases the lifespan of C. elegans via skn-1 activation. / Powolny, Anna A.; Singh, Shivendra V.; Melov, Simon; Hubbard, Alan; Fisher, Alfred L.

In: Experimental Gerontology, Vol. 46, No. 6, 01.06.2011, p. 441-452.

Research output: Contribution to journalArticle

Powolny, Anna A. ; Singh, Shivendra V. ; Melov, Simon ; Hubbard, Alan ; Fisher, Alfred L. / The garlic constituent diallyl trisulfide increases the lifespan of C. elegans via skn-1 activation. In: Experimental Gerontology. 2011 ; Vol. 46, No. 6. pp. 441-452.
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