The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis

Bin Yu, Liu Bi, Binglian Zheng, Lijuan Ji, David Chevalier, Manu Agarwal, Vanitharani Ramachandran, Wanxiang Li, Thierry Lagrange, John C. Walker, Xuemei Chen

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Proteins containing the forkhead-associated domain (FHA) are known to act in biological processes such as DNA damage repair, protein degradation, and signal transduction. Here we report that DAWDLE (DDL), an FHA domain-containing protein in Arabidopsis, acts in the biogenesis of miRNAs and endogenous siRNAs. Unlike mutants of genes known to participate in the processing of miRNA precursors, such as dcl1, hyponastic leaves1, and serrate, ddl mutants show reduced levels of pri-miRNAs as well as mature miRNAs. Promoter activity of MIR genes, however, is not affected by ddl mutations. DDL is an RNA binding protein and is able to interact with DCL1. In addition, we found that SNIP1, the human homolog of DDL, is involved in miRNA biogenesis and interacts with Drosha. Therefore, we uncovered an evolutionarily conserved factor in miRNA biogenesis. We propose that DDL participates in miRNA biogenesis by facilitating DCL1 to access or recognize pri-miRNAs.

Original languageEnglish (US)
Pages (from-to)10073-10078
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number29
DOIs
StatePublished - Jul 22 2008

Fingerprint

MicroRNAs
RNA
Arabidopsis Proteins
Forkhead Transcription Factors
Biological Phenomena
RNA-Binding Proteins
Protein Domains
Arabidopsis DAWDLE protein
DNA Repair
Proteolysis
Genes
DNA Damage
Signal Transduction
Mutation

Keywords

  • DCL1
  • Drosha
  • SMAD
  • microRNA
  • siRNA

ASJC Scopus subject areas

  • General

Cite this

The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis. / Yu, Bin; Bi, Liu; Zheng, Binglian; Ji, Lijuan; Chevalier, David; Agarwal, Manu; Ramachandran, Vanitharani; Li, Wanxiang; Lagrange, Thierry; Walker, John C.; Chen, Xuemei.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 29, 22.07.2008, p. 10073-10078.

Research output: Contribution to journalArticle

Yu, B, Bi, L, Zheng, B, Ji, L, Chevalier, D, Agarwal, M, Ramachandran, V, Li, W, Lagrange, T, Walker, JC & Chen, X 2008, 'The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 29, pp. 10073-10078. https://doi.org/10.1073/pnas.0804218105
Yu, Bin ; Bi, Liu ; Zheng, Binglian ; Ji, Lijuan ; Chevalier, David ; Agarwal, Manu ; Ramachandran, Vanitharani ; Li, Wanxiang ; Lagrange, Thierry ; Walker, John C. ; Chen, Xuemei. / The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 29. pp. 10073-10078.
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