The FcγRI receptor signals through the activation of hck and MAP kinase

Donald L. Durden, Hwang Min Kim, Barbara Calore, Yenbou Liu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

U937 cells differentiated with IFN-γ (termed U937IF cells) were used to study FcγRI signaling. IFN induces a functional FcγRI receptor signaling pathway in U937 cells, leading to the activation of the respiratory burst. IFN induces the expression of the nonreceptor protein tyrosine kinase, hck, and cross-linking the FcγRI receptor in U937IF cells results in the activation of hck kinase as evidenced by the three- to fivefold increased tyrosine phosphorylation of hck. In vitro kinase assays demonstrate that the specific kinase activity of hck is increased 10-fold after FcγRI stimulation, hck is observed to associate with two prominent tyrosine-phosphorylated proteins, p72 and p95, after FcγRI-activation. FcγRI cross-linking also results in mobility shift in MAP kinase in U937IF cells, suggesting that the FcγRI receptor signals through the activation of MAP kinase. The data suggest that hck, p72, p95, and MAP kinase are involved in signal transduction through the FcγRI receptor.

Original languageEnglish (US)
Pages (from-to)4048-4054
Number of pages7
JournalJournal of Immunology
Volume154
Issue number8
StatePublished - Dec 1 1995

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Phosphotransferases
U937 Cells
Tyrosine
Respiratory Burst
Protein-Tyrosine Kinases
Signal Transduction
Phosphorylation
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Durden, D. L., Kim, H. M., Calore, B., & Liu, Y. (1995). The FcγRI receptor signals through the activation of hck and MAP kinase. Journal of Immunology, 154(8), 4048-4054.

The FcγRI receptor signals through the activation of hck and MAP kinase. / Durden, Donald L.; Kim, Hwang Min; Calore, Barbara; Liu, Yenbou.

In: Journal of Immunology, Vol. 154, No. 8, 01.12.1995, p. 4048-4054.

Research output: Contribution to journalArticle

Durden, DL, Kim, HM, Calore, B & Liu, Y 1995, 'The FcγRI receptor signals through the activation of hck and MAP kinase', Journal of Immunology, vol. 154, no. 8, pp. 4048-4054.
Durden DL, Kim HM, Calore B, Liu Y. The FcγRI receptor signals through the activation of hck and MAP kinase. Journal of Immunology. 1995 Dec 1;154(8):4048-4054.
Durden, Donald L. ; Kim, Hwang Min ; Calore, Barbara ; Liu, Yenbou. / The FcγRI receptor signals through the activation of hck and MAP kinase. In: Journal of Immunology. 1995 ; Vol. 154, No. 8. pp. 4048-4054.
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