The fadL gene product of Escherichia coli is an outer membrane protein required for uptake of long-chain fatty acids and involved in sensitivity to bacteriophage T2

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Abstract

The fadL+ gene of Escherichia coli encodes an outer membrane protein (FadL) essential for the uptake of long-chain fatty acids (C12 to C18). The present study shows that in addition to being required for uptake of and growth on the long-chain fatty acid oleate (C(18:1)), FadL acts as a receptor of bacteriophage T2. Bacteriophage T2-resistant (T2(r)) strains lacked FadL and were unable to take up and grow on long-chain fatty acids. Upon transformation with the fadL+ clone pN103, T2(r) strains became sensitive to bacteriophage T2 (T2(s)), became able to take up long-chain fatty acids at wild-type levels, and contained FadL in the outer membrane.

Original languageEnglish (US)
Pages (from-to)2850-2854
Number of pages5
JournalJournal of bacteriology
Volume170
Issue number6
DOIs
StatePublished - Jan 1 1988

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Bacteriophage T4
Membrane Proteins
Fatty Acids
Escherichia coli
Genes
Oleic Acid
Clone Cells
Membranes
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

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abstract = "The fadL+ gene of Escherichia coli encodes an outer membrane protein (FadL) essential for the uptake of long-chain fatty acids (C12 to C18). The present study shows that in addition to being required for uptake of and growth on the long-chain fatty acid oleate (C(18:1)), FadL acts as a receptor of bacteriophage T2. Bacteriophage T2-resistant (T2(r)) strains lacked FadL and were unable to take up and grow on long-chain fatty acids. Upon transformation with the fadL+ clone pN103, T2(r) strains became sensitive to bacteriophage T2 (T2(s)), became able to take up long-chain fatty acids at wild-type levels, and contained FadL in the outer membrane.",
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N2 - The fadL+ gene of Escherichia coli encodes an outer membrane protein (FadL) essential for the uptake of long-chain fatty acids (C12 to C18). The present study shows that in addition to being required for uptake of and growth on the long-chain fatty acid oleate (C(18:1)), FadL acts as a receptor of bacteriophage T2. Bacteriophage T2-resistant (T2(r)) strains lacked FadL and were unable to take up and grow on long-chain fatty acids. Upon transformation with the fadL+ clone pN103, T2(r) strains became sensitive to bacteriophage T2 (T2(s)), became able to take up long-chain fatty acids at wild-type levels, and contained FadL in the outer membrane.

AB - The fadL+ gene of Escherichia coli encodes an outer membrane protein (FadL) essential for the uptake of long-chain fatty acids (C12 to C18). The present study shows that in addition to being required for uptake of and growth on the long-chain fatty acid oleate (C(18:1)), FadL acts as a receptor of bacteriophage T2. Bacteriophage T2-resistant (T2(r)) strains lacked FadL and were unable to take up and grow on long-chain fatty acids. Upon transformation with the fadL+ clone pN103, T2(r) strains became sensitive to bacteriophage T2 (T2(s)), became able to take up long-chain fatty acids at wild-type levels, and contained FadL in the outer membrane.

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