The expression of multidrug resistance-associated protein (MRP) in pancreatic adenocarcinoma cell lines

Donald W. Miller, Michelle Fontain, Carol Kolar, Terence Lawson

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

The presence of P-glycoprotein (P-gp) and multiple drug resistance-associated protein (MRP) was examined in four human pancreatic adenocarcinoma cell lines (PANC-1, BxPC-3, AsPC-1, and Capan-1). Cellular accumulation of rhodamine 123 and [3H]vincristine were used to determine functional activity of P-gp and MRP, respectively. None of the cells showed any evidence of P-gp in the rhodamine 123 cellular accumulation assays. In contrast, PANC-1, BxPC-3 and AsPC-1 did display an increased accumulation of [3H]vincristine following treatment with either cyclosporin A or verapamil. Western blot analysis confirmed the expression of MRP, and little, if any, measurable P-gp in the cell lysates. These studies suggest that intrinsic drug resistance in pancreatic duct cancer may be due in part to the presence of MRP.

Original languageEnglish (US)
Pages (from-to)301-306
Number of pages6
JournalCancer Letters
Volume107
Issue number2
DOIs
StatePublished - Oct 22 1996

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Keywords

  • Drug resistance
  • MRP
  • P-gp
  • Pancreatic adenocarcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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