The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine

Alessandro Achilli, Nabil Matmati, Enrico Casalone, Giorgio Morpurgo, Angela Lucaccioni, Youri I Pavlov, Nora Babudri

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Mutagenesis induced in the yeast Saccharomyces cerevisiae by starvation for nutrilites is a well-documented phenomenon of an unknown mechanism. We have previously shown that the polymerase delta proofreading activity controls spontaneous mutagenesis in cells starved for histidine. To obtain further information, we compared the effect of adenine starvation on mutagenesis in wild-type cells and, in cells lacking the proofreading activity of polymerase delta (phenotype Exo-, mutation pol3-01). Results: Ade+ revertants accumulated at a very high rate on adenine-free plates so that their frequency on day 16 after plating was 1.5 × 10-4 for wild-type and 1.0 × 10-2 for the Exo- strain. In the Exo- strain, all revertants arising under adenine starvation are suppressors of the original mutation, most possessed additional nutritional requirements, and 50% of them were temperature sensitive. Conclusions: Adenine starvation is highly mutagenic in yeast. The deficiency in the polymerase delta proofreading activity in strains with the pol3-01 mutation leads to a further 66-fold increase of the rate of mutations. Our data suggest that adenine starvation induces genome-wide hypermutagenesis in the Exo- strain.

Original languageEnglish (US)
Article number34
JournalBMC Genetics
Volume5
DOIs
StatePublished - Dec 23 2004

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Exonucleases
Adenine
Mutation Rate
Starvation
Saccharomyces cerevisiae
Mutagenesis
Yeasts
Genetic Suppression
Nutritional Requirements
Mutation
Histidine
Genome
Phenotype
Temperature

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine. / Achilli, Alessandro; Matmati, Nabil; Casalone, Enrico; Morpurgo, Giorgio; Lucaccioni, Angela; Pavlov, Youri I; Babudri, Nora.

In: BMC Genetics, Vol. 5, 34, 23.12.2004.

Research output: Contribution to journalArticle

Achilli, Alessandro ; Matmati, Nabil ; Casalone, Enrico ; Morpurgo, Giorgio ; Lucaccioni, Angela ; Pavlov, Youri I ; Babudri, Nora. / The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine. In: BMC Genetics. 2004 ; Vol. 5.
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AU - Morpurgo, Giorgio

AU - Lucaccioni, Angela

AU - Pavlov, Youri I

AU - Babudri, Nora

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N2 - Background: Mutagenesis induced in the yeast Saccharomyces cerevisiae by starvation for nutrilites is a well-documented phenomenon of an unknown mechanism. We have previously shown that the polymerase delta proofreading activity controls spontaneous mutagenesis in cells starved for histidine. To obtain further information, we compared the effect of adenine starvation on mutagenesis in wild-type cells and, in cells lacking the proofreading activity of polymerase delta (phenotype Exo-, mutation pol3-01). Results: Ade+ revertants accumulated at a very high rate on adenine-free plates so that their frequency on day 16 after plating was 1.5 × 10-4 for wild-type and 1.0 × 10-2 for the Exo- strain. In the Exo- strain, all revertants arising under adenine starvation are suppressors of the original mutation, most possessed additional nutritional requirements, and 50% of them were temperature sensitive. Conclusions: Adenine starvation is highly mutagenic in yeast. The deficiency in the polymerase delta proofreading activity in strains with the pol3-01 mutation leads to a further 66-fold increase of the rate of mutations. Our data suggest that adenine starvation induces genome-wide hypermutagenesis in the Exo- strain.

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