The ESCRT-I Subunit TSG101 controls endosome-to-cytosol release of viral RNA

Pierre Philippe Luyet, Thomas Falguières, Véronique Pons, Asit K Pattnaik, Jean Gruenberg

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Like other enveloped viruses, vesicular stomatitis virus infects cells through endosomes. There, the viral envelope undergoes fusion with endosomal membranes, thereby releasing the nucleocapsid into the cytoplasm and allowing infection to proceed. Previously, we reported that the viral envelope fuses preferentially with the membrane of vesicles present within multivesicular endosomes. Then, these intra-endosomal vesicles (containing nucleocapsids) are transported to late endosomes, where back-fusion with the endosome limiting membrane delivers the nucleocapsid into the cytoplasm. In this study, we show that the tumor susceptibility gene 101 (Tsg101) subunit of the endosomal sorting complexes required for transport (ESCRT)-I complex, which mediates receptor sorting into multivesicular endosomes, is dispensable for viral envelope fusion with endosomal membranes and viral RNA transport to late endosomes but is necessary for infection. Our data indicate that Tsg101, in contrast to the ESCRT-0 component Hrs, plays a direct role in nucleocapsid release from within multivesicular endosomes to the cytoplasm, presumably by controlling the back-fusion process. We conclude that Tsg101, through selective interactions with its partners including Hrs and Alix, may link receptor sorting and lysosome targeting to the back-fusion process involved in viral capsid release.

Original languageEnglish (US)
Pages (from-to)2279-2290
Number of pages12
JournalTraffic
Volume9
Issue number12
DOIs
StatePublished - Nov 20 2008

Fingerprint

Endosomal Sorting Complexes Required for Transport
Endosomes
Viral RNA
Nucleocapsid
Cytosol
Fusion reactions
Membranes
Viruses
Sorting
Cytoplasm
Electric fuses
RNA Transport
Vesicular Stomatitis
Capsid
Lysosomes
Infection
Tsg101 protein

Keywords

  • ESCRTs
  • Endocytosis
  • Enveloped virus
  • Intraendosomal transport
  • Multivesicular body
  • Tsg101
  • Vesicular stomatitis virus

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The ESCRT-I Subunit TSG101 controls endosome-to-cytosol release of viral RNA. / Luyet, Pierre Philippe; Falguières, Thomas; Pons, Véronique; Pattnaik, Asit K; Gruenberg, Jean.

In: Traffic, Vol. 9, No. 12, 20.11.2008, p. 2279-2290.

Research output: Contribution to journalArticle

Luyet, Pierre Philippe ; Falguières, Thomas ; Pons, Véronique ; Pattnaik, Asit K ; Gruenberg, Jean. / The ESCRT-I Subunit TSG101 controls endosome-to-cytosol release of viral RNA. In: Traffic. 2008 ; Vol. 9, No. 12. pp. 2279-2290.
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