The ERK and PI3K signaling pathways mediate inhibition of insulin-like growth factor-1 receptor mRNA expression by somatostatin

Andrea Hanson, Deepak Poudyal, Alison Hagemeister, Katie Reindl, Mark A. Sheridan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Somatostatins (SSs) are a structurally diverse family of peptide hormones that regulate various aspects of growth, development, and metabolism in vertebrates. Previously, we showed that SSs inhibit mRNA and functional expression of insulin-like growth factor-1 receptors (IGFR1) in gill filaments of rainbow trout. In this study, we used trout gill filaments, which express in high abundance two distinct IGFR1s, IGFR1A and IGFR1B, to examine the mechanism(s) through which SSs exert their inhibitory effects on IGFR1 expression. SS-14, a predominat SS isoform, directly stimulated the phosphorylation of extracellular signal-regulated kinase (ERK) and protein kinase B (Akt), a downstream target of phosphatidylinositol 3-kinase (PI3K), in filaments incubated in vitro. Activation of ERK and Akt by SS-14 was rapid, occuring within 5-10 min, and was concentration-dependent. The ERK pathway inhibitor, U0126, retarded SS-14-stimulated phosphorylation of ERK 1/2, whereas the PI3K inhibitor, LY294002, blocked SS-14-stimulated phosphorylation of Akt. SS-14-inhibited expression of IGFR1 mRNAs was blocked by both U0126 and LY294002. These data indicate that SS-14 inhibition of IGFR1 mRNA expression is mediated through the ERK and PI3K/Akt signaling pathways.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume315
Issue number1-2
DOIs
StatePublished - Feb 5 2010

Fingerprint

Phosphatidylinositol 3-Kinase
Somatomedin Receptors
Extracellular Signal-Regulated MAP Kinases
Somatomedins
Somatostatin
Messenger RNA
Phosphorylation
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Proto-Oncogene Proteins c-akt
Mitogen-Activated Protein Kinase 3
Peptide Hormones
Trout
Oncorhynchus mykiss
Mitogen-Activated Protein Kinase 1
Growth and Development
Metabolism
Vertebrates
Protein Isoforms
Chemical activation

Keywords

  • Akt
  • LY294002
  • MAPK
  • Rainbow trout
  • U0126

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

The ERK and PI3K signaling pathways mediate inhibition of insulin-like growth factor-1 receptor mRNA expression by somatostatin. / Hanson, Andrea; Poudyal, Deepak; Hagemeister, Alison; Reindl, Katie; Sheridan, Mark A.

In: Molecular and Cellular Endocrinology, Vol. 315, No. 1-2, 05.02.2010, p. 57-62.

Research output: Contribution to journalArticle

Hanson, Andrea ; Poudyal, Deepak ; Hagemeister, Alison ; Reindl, Katie ; Sheridan, Mark A. / The ERK and PI3K signaling pathways mediate inhibition of insulin-like growth factor-1 receptor mRNA expression by somatostatin. In: Molecular and Cellular Endocrinology. 2010 ; Vol. 315, No. 1-2. pp. 57-62.
@article{34b91842be584a1398673718095a404b,
title = "The ERK and PI3K signaling pathways mediate inhibition of insulin-like growth factor-1 receptor mRNA expression by somatostatin",
abstract = "Somatostatins (SSs) are a structurally diverse family of peptide hormones that regulate various aspects of growth, development, and metabolism in vertebrates. Previously, we showed that SSs inhibit mRNA and functional expression of insulin-like growth factor-1 receptors (IGFR1) in gill filaments of rainbow trout. In this study, we used trout gill filaments, which express in high abundance two distinct IGFR1s, IGFR1A and IGFR1B, to examine the mechanism(s) through which SSs exert their inhibitory effects on IGFR1 expression. SS-14, a predominat SS isoform, directly stimulated the phosphorylation of extracellular signal-regulated kinase (ERK) and protein kinase B (Akt), a downstream target of phosphatidylinositol 3-kinase (PI3K), in filaments incubated in vitro. Activation of ERK and Akt by SS-14 was rapid, occuring within 5-10 min, and was concentration-dependent. The ERK pathway inhibitor, U0126, retarded SS-14-stimulated phosphorylation of ERK 1/2, whereas the PI3K inhibitor, LY294002, blocked SS-14-stimulated phosphorylation of Akt. SS-14-inhibited expression of IGFR1 mRNAs was blocked by both U0126 and LY294002. These data indicate that SS-14 inhibition of IGFR1 mRNA expression is mediated through the ERK and PI3K/Akt signaling pathways.",
keywords = "Akt, LY294002, MAPK, Rainbow trout, U0126",
author = "Andrea Hanson and Deepak Poudyal and Alison Hagemeister and Katie Reindl and Sheridan, {Mark A.}",
year = "2010",
month = "2",
day = "5",
doi = "10.1016/j.mce.2009.09.034",
language = "English (US)",
volume = "315",
pages = "57--62",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - The ERK and PI3K signaling pathways mediate inhibition of insulin-like growth factor-1 receptor mRNA expression by somatostatin

AU - Hanson, Andrea

AU - Poudyal, Deepak

AU - Hagemeister, Alison

AU - Reindl, Katie

AU - Sheridan, Mark A.

PY - 2010/2/5

Y1 - 2010/2/5

N2 - Somatostatins (SSs) are a structurally diverse family of peptide hormones that regulate various aspects of growth, development, and metabolism in vertebrates. Previously, we showed that SSs inhibit mRNA and functional expression of insulin-like growth factor-1 receptors (IGFR1) in gill filaments of rainbow trout. In this study, we used trout gill filaments, which express in high abundance two distinct IGFR1s, IGFR1A and IGFR1B, to examine the mechanism(s) through which SSs exert their inhibitory effects on IGFR1 expression. SS-14, a predominat SS isoform, directly stimulated the phosphorylation of extracellular signal-regulated kinase (ERK) and protein kinase B (Akt), a downstream target of phosphatidylinositol 3-kinase (PI3K), in filaments incubated in vitro. Activation of ERK and Akt by SS-14 was rapid, occuring within 5-10 min, and was concentration-dependent. The ERK pathway inhibitor, U0126, retarded SS-14-stimulated phosphorylation of ERK 1/2, whereas the PI3K inhibitor, LY294002, blocked SS-14-stimulated phosphorylation of Akt. SS-14-inhibited expression of IGFR1 mRNAs was blocked by both U0126 and LY294002. These data indicate that SS-14 inhibition of IGFR1 mRNA expression is mediated through the ERK and PI3K/Akt signaling pathways.

AB - Somatostatins (SSs) are a structurally diverse family of peptide hormones that regulate various aspects of growth, development, and metabolism in vertebrates. Previously, we showed that SSs inhibit mRNA and functional expression of insulin-like growth factor-1 receptors (IGFR1) in gill filaments of rainbow trout. In this study, we used trout gill filaments, which express in high abundance two distinct IGFR1s, IGFR1A and IGFR1B, to examine the mechanism(s) through which SSs exert their inhibitory effects on IGFR1 expression. SS-14, a predominat SS isoform, directly stimulated the phosphorylation of extracellular signal-regulated kinase (ERK) and protein kinase B (Akt), a downstream target of phosphatidylinositol 3-kinase (PI3K), in filaments incubated in vitro. Activation of ERK and Akt by SS-14 was rapid, occuring within 5-10 min, and was concentration-dependent. The ERK pathway inhibitor, U0126, retarded SS-14-stimulated phosphorylation of ERK 1/2, whereas the PI3K inhibitor, LY294002, blocked SS-14-stimulated phosphorylation of Akt. SS-14-inhibited expression of IGFR1 mRNAs was blocked by both U0126 and LY294002. These data indicate that SS-14 inhibition of IGFR1 mRNA expression is mediated through the ERK and PI3K/Akt signaling pathways.

KW - Akt

KW - LY294002

KW - MAPK

KW - Rainbow trout

KW - U0126

UR - http://www.scopus.com/inward/record.url?scp=71849105524&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71849105524&partnerID=8YFLogxK

U2 - 10.1016/j.mce.2009.09.034

DO - 10.1016/j.mce.2009.09.034

M3 - Article

VL - 315

SP - 57

EP - 62

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 1-2

ER -