The Entner-Doudoroff pathway in Escherichia coli is induced for oxidative glucose metabolism via pyrroloquinoline quinone-dependent glucose dehydrogenase

R. Fliege, S. Tong, A. Shibata, K. W. Nickerson, T. Conway

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The Entner-Doudoroff pathway was shown to be induced for oxidative glucose metabolism when Escherichia coli was provided with the periplasmic glucose dehydrogenase cofactor pyrroloquinoline quinone (PQQ). Induction of the Entner-Doudoroff pathway by glucose plus PQQ was established both genetically and biochemically and was shown to occur in glucose transport mutants, as well as in wild-type E. coli. These data complete the body of evidence that proves the existence of a pathway for oxidative glucose metabolism in E. coli. PQQ-dependent oxidative glucose metabolism provides a metabolic branch point in the periplasm; the choices are either oxidation to gluconate followed by induction of the Entner-Doudoroff pathway or phosphotransferase- mediated transport. The oxidative glucose pathway might be important for survival of enteric bacteria in aerobic, low-phosphate, aquatic environments.

Original languageEnglish (US)
Pages (from-to)3826-3829
Number of pages4
JournalApplied and environmental microbiology
Volume58
Issue number12
StatePublished - Jan 1 1992

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glucose dehydrogenase (pyrroloquinoline-quinone)
quinones
glucose
metabolism
Escherichia coli
Glucose
PQQ Cofactor
Periplasm
Enterobacteriaceae
aquatic environment
intestinal microorganisms
Phosphotransferases
Phosphates

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

The Entner-Doudoroff pathway in Escherichia coli is induced for oxidative glucose metabolism via pyrroloquinoline quinone-dependent glucose dehydrogenase. / Fliege, R.; Tong, S.; Shibata, A.; Nickerson, K. W.; Conway, T.

In: Applied and environmental microbiology, Vol. 58, No. 12, 01.01.1992, p. 3826-3829.

Research output: Contribution to journalArticle

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