The endothelial lipase protein is promising urinary biomarker for diagnosis of gastric cancer

Xueyan Dong, Guoqing Wang, Guoqing Zhang, Zhaohui Ni, Jian Suo, Juan Cui, Ai Cui, Qing Yang, Ying Xu, Fan Li

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Gastric cancer is one of the most common malignant tumors in the world. Finding effective diagnostic biomarkers in urine or serum would represent the most ideal solution to detecting gastric cancer during annual physical examination. This study was to evaluate the potential of endothelial lipase (EL) as a urinary biomarker for diagnosis of gastric cancer.Methods: The expression levels of EL was measured using Western blotting and immunohistochemical staining experiments on (tissue, serum, and urine) samples of gastric cancer patients versus healthy people. We also checked the EL levels in the urine samples of other cancer types (lung, colon and rectum cancers) and benign lesions (gastritis and gastric leiomyoma) to check if EL was specific to gastric cancer.Result: We observed a clear separation between the EL expression levels in the urine samples of 90 gastric cancer patients and of 57 healthy volunteers. It was approximately 9.9 fold average decrease of the EL expression levels in the urine samples of gastric cancer compared to the healthy controls (P <0.0001), achieving a 0.967 AUC value for the ROC (receiver operating characteristic) curve, demonstrating it's highly accurate as a diagnostic marker for gastric cancer. Interestingly, the expression levels of EL in tissue and serum samples were not nearly as discriminative as in urine samples (P = 0.90 and P = 0.79). In immunohistochemical experiments, positive expression of the EL protein was found in 67% (8/12) of gastric adjacent noncancerous and in 58% (7/12) of gastric cancer samples. There was no significant statistical in the expression levels of this protein between the gastric cancer and the matching noncancerous tissues (P =0.67).Conclusions: The urinary EL as a highly accurate gastric cancer biomarker that is potentially applicable to the general screening with high sensitivity and specificity.Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4527331618757552.

Original languageEnglish (US)
Article number45
JournalDiagnostic Pathology
Volume8
Issue number1
DOIs
StatePublished - Mar 19 2013

Fingerprint

Lipase
Stomach Neoplasms
Biomarkers
Proteins
Urine
Rectal Neoplasms
Colonic Neoplasms
Lung Neoplasms
Stomach
Serum
Leiomyoma
Gastritis
Tumor Biomarkers
ROC Curve
Physical Examination
Area Under Curve
Healthy Volunteers
Western Blotting
Staining and Labeling
Sensitivity and Specificity

Keywords

  • Biomarker
  • Diagnosis
  • Endothelial lipase
  • Gastric cancer

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

The endothelial lipase protein is promising urinary biomarker for diagnosis of gastric cancer. / Dong, Xueyan; Wang, Guoqing; Zhang, Guoqing; Ni, Zhaohui; Suo, Jian; Cui, Juan; Cui, Ai; Yang, Qing; Xu, Ying; Li, Fan.

In: Diagnostic Pathology, Vol. 8, No. 1, 45, 19.03.2013.

Research output: Contribution to journalArticle

Dong, Xueyan ; Wang, Guoqing ; Zhang, Guoqing ; Ni, Zhaohui ; Suo, Jian ; Cui, Juan ; Cui, Ai ; Yang, Qing ; Xu, Ying ; Li, Fan. / The endothelial lipase protein is promising urinary biomarker for diagnosis of gastric cancer. In: Diagnostic Pathology. 2013 ; Vol. 8, No. 1.
@article{0192fc05157444b2a6b123332ba91506,
title = "The endothelial lipase protein is promising urinary biomarker for diagnosis of gastric cancer",
abstract = "Background: Gastric cancer is one of the most common malignant tumors in the world. Finding effective diagnostic biomarkers in urine or serum would represent the most ideal solution to detecting gastric cancer during annual physical examination. This study was to evaluate the potential of endothelial lipase (EL) as a urinary biomarker for diagnosis of gastric cancer.Methods: The expression levels of EL was measured using Western blotting and immunohistochemical staining experiments on (tissue, serum, and urine) samples of gastric cancer patients versus healthy people. We also checked the EL levels in the urine samples of other cancer types (lung, colon and rectum cancers) and benign lesions (gastritis and gastric leiomyoma) to check if EL was specific to gastric cancer.Result: We observed a clear separation between the EL expression levels in the urine samples of 90 gastric cancer patients and of 57 healthy volunteers. It was approximately 9.9 fold average decrease of the EL expression levels in the urine samples of gastric cancer compared to the healthy controls (P <0.0001), achieving a 0.967 AUC value for the ROC (receiver operating characteristic) curve, demonstrating it's highly accurate as a diagnostic marker for gastric cancer. Interestingly, the expression levels of EL in tissue and serum samples were not nearly as discriminative as in urine samples (P = 0.90 and P = 0.79). In immunohistochemical experiments, positive expression of the EL protein was found in 67{\%} (8/12) of gastric adjacent noncancerous and in 58{\%} (7/12) of gastric cancer samples. There was no significant statistical in the expression levels of this protein between the gastric cancer and the matching noncancerous tissues (P =0.67).Conclusions: The urinary EL as a highly accurate gastric cancer biomarker that is potentially applicable to the general screening with high sensitivity and specificity.Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4527331618757552.",
keywords = "Biomarker, Diagnosis, Endothelial lipase, Gastric cancer",
author = "Xueyan Dong and Guoqing Wang and Guoqing Zhang and Zhaohui Ni and Jian Suo and Juan Cui and Ai Cui and Qing Yang and Ying Xu and Fan Li",
year = "2013",
month = "3",
day = "19",
doi = "10.1186/1746-1596-8-45",
language = "English (US)",
volume = "8",
journal = "Diagnostic Pathology",
issn = "1746-1596",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - The endothelial lipase protein is promising urinary biomarker for diagnosis of gastric cancer

AU - Dong, Xueyan

AU - Wang, Guoqing

AU - Zhang, Guoqing

AU - Ni, Zhaohui

AU - Suo, Jian

AU - Cui, Juan

AU - Cui, Ai

AU - Yang, Qing

AU - Xu, Ying

AU - Li, Fan

PY - 2013/3/19

Y1 - 2013/3/19

N2 - Background: Gastric cancer is one of the most common malignant tumors in the world. Finding effective diagnostic biomarkers in urine or serum would represent the most ideal solution to detecting gastric cancer during annual physical examination. This study was to evaluate the potential of endothelial lipase (EL) as a urinary biomarker for diagnosis of gastric cancer.Methods: The expression levels of EL was measured using Western blotting and immunohistochemical staining experiments on (tissue, serum, and urine) samples of gastric cancer patients versus healthy people. We also checked the EL levels in the urine samples of other cancer types (lung, colon and rectum cancers) and benign lesions (gastritis and gastric leiomyoma) to check if EL was specific to gastric cancer.Result: We observed a clear separation between the EL expression levels in the urine samples of 90 gastric cancer patients and of 57 healthy volunteers. It was approximately 9.9 fold average decrease of the EL expression levels in the urine samples of gastric cancer compared to the healthy controls (P <0.0001), achieving a 0.967 AUC value for the ROC (receiver operating characteristic) curve, demonstrating it's highly accurate as a diagnostic marker for gastric cancer. Interestingly, the expression levels of EL in tissue and serum samples were not nearly as discriminative as in urine samples (P = 0.90 and P = 0.79). In immunohistochemical experiments, positive expression of the EL protein was found in 67% (8/12) of gastric adjacent noncancerous and in 58% (7/12) of gastric cancer samples. There was no significant statistical in the expression levels of this protein between the gastric cancer and the matching noncancerous tissues (P =0.67).Conclusions: The urinary EL as a highly accurate gastric cancer biomarker that is potentially applicable to the general screening with high sensitivity and specificity.Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4527331618757552.

AB - Background: Gastric cancer is one of the most common malignant tumors in the world. Finding effective diagnostic biomarkers in urine or serum would represent the most ideal solution to detecting gastric cancer during annual physical examination. This study was to evaluate the potential of endothelial lipase (EL) as a urinary biomarker for diagnosis of gastric cancer.Methods: The expression levels of EL was measured using Western blotting and immunohistochemical staining experiments on (tissue, serum, and urine) samples of gastric cancer patients versus healthy people. We also checked the EL levels in the urine samples of other cancer types (lung, colon and rectum cancers) and benign lesions (gastritis and gastric leiomyoma) to check if EL was specific to gastric cancer.Result: We observed a clear separation between the EL expression levels in the urine samples of 90 gastric cancer patients and of 57 healthy volunteers. It was approximately 9.9 fold average decrease of the EL expression levels in the urine samples of gastric cancer compared to the healthy controls (P <0.0001), achieving a 0.967 AUC value for the ROC (receiver operating characteristic) curve, demonstrating it's highly accurate as a diagnostic marker for gastric cancer. Interestingly, the expression levels of EL in tissue and serum samples were not nearly as discriminative as in urine samples (P = 0.90 and P = 0.79). In immunohistochemical experiments, positive expression of the EL protein was found in 67% (8/12) of gastric adjacent noncancerous and in 58% (7/12) of gastric cancer samples. There was no significant statistical in the expression levels of this protein between the gastric cancer and the matching noncancerous tissues (P =0.67).Conclusions: The urinary EL as a highly accurate gastric cancer biomarker that is potentially applicable to the general screening with high sensitivity and specificity.Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4527331618757552.

KW - Biomarker

KW - Diagnosis

KW - Endothelial lipase

KW - Gastric cancer

UR - http://www.scopus.com/inward/record.url?scp=84875041393&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875041393&partnerID=8YFLogxK

U2 - 10.1186/1746-1596-8-45

DO - 10.1186/1746-1596-8-45

M3 - Article

VL - 8

JO - Diagnostic Pathology

JF - Diagnostic Pathology

SN - 1746-1596

IS - 1

M1 - 45

ER -