The effects of laminin on the growth and differentiation of embryonal carcinoma cells in defined media

A. Rizzino, V. Terranova, D. Rohrbach, C. Crowley, H. Rizzino

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In this paper we have examined the growth and differentiation of the embryonal carcinoma cell line, F9, in the defined medium EM-3 at low density. We show that the growth of F9 and their differentiated cells (F9-diff) in EM-3 is strongly density dependent. At low cell densities the growth of both cell types is severely limited and most of the cells do not survive. Although this poses a problem for working with F9 and F9-diff in EM-3, it provides a convenient assay for identifying molecules that support their growth at low density. Using this assay, we have determined that laminin, a newly isolated glycoprotein of basement membranes, significantly improves the growth and short-term survival of both F9 and F9-diff. However, addition of laminin to EM-3 is insufficient to promote the clonal growth of these cell types. Our findings also indicate that laminin promotes the attachment of F9 and F9-diff in defined media. On the basis of our results, we propose an attachment function for laminin during the early stages of mammalian development.

Original languageEnglish (US)
Pages (from-to)243-253
Number of pages11
JournalUnknown Journal
Volume13
Issue number2
DOIs
StatePublished - Jan 1 1980

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Embryonal Carcinoma Stem Cells
Laminin
Cells
Growth
Assays
Basement Membrane
Glycoproteins
Cell Count
Cell Line
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

The effects of laminin on the growth and differentiation of embryonal carcinoma cells in defined media. / Rizzino, A.; Terranova, V.; Rohrbach, D.; Crowley, C.; Rizzino, H.

In: Unknown Journal, Vol. 13, No. 2, 01.01.1980, p. 243-253.

Research output: Contribution to journalArticle

Rizzino, A. ; Terranova, V. ; Rohrbach, D. ; Crowley, C. ; Rizzino, H. / The effects of laminin on the growth and differentiation of embryonal carcinoma cells in defined media. In: Unknown Journal. 1980 ; Vol. 13, No. 2. pp. 243-253.
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