The Effects of Interferon-γ and Bacterial Lipopolysaccharide on CD14 Expression in Human Monocytes

Jeffrey B Payne, F. C. Nichols, J. F. Peluso

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

CD14 has been reported to be the lipopolysaccharide (LPS)-LPS binding protein receptor. The effects of interferon-γ (IFN-γ) on CD14 expression have not been clearly established. The purpose of this investigation was to examine the effects of IFN-γ alone and IFN-γ followed by bacterial LPS on CD14 expression. Human peripheral blood monocytes were isolated by counterflow centrifugal elutriation (CCE). Monocytes were cultured for 48 h with IFN-γ alone or for 24 h with IFN-γ followed by LPS for a second 24 h. IFN-γ alone caused a down-regulation of CD14 expression, as assessed by flow cytometry, relative to CD14 expression in untreated monocytes. In addition, CD14 expression was even more significantly down-regulated after IFN-γ pretreatment followed by either Prevotella intermedia or Salmonella typhimurium LPS. Likewise, the percentage of CD14+ monocytes decreased after IFN-γ alone and even more dramatically after IFN-γ treatment followed by either LPS. This study clearly demonstrated that IFN-γ down-regulates CD14 expression and that LPS following IFN-γ pretreatment potentiates this effect.

Original languageEnglish (US)
Pages (from-to)307-310
Number of pages4
JournalJournal of Interferon Research
Volume12
Issue number4
DOIs
StatePublished - Aug 1992

Fingerprint

Interferons
Lipopolysaccharides
Monocytes
Down-Regulation
Prevotella intermedia
Salmonella typhimurium
Flow Cytometry

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

The Effects of Interferon-γ and Bacterial Lipopolysaccharide on CD14 Expression in Human Monocytes. / Payne, Jeffrey B; Nichols, F. C.; Peluso, J. F.

In: Journal of Interferon Research, Vol. 12, No. 4, 08.1992, p. 307-310.

Research output: Contribution to journalArticle

@article{b0b8c51094254b81b4484fbaab7af9d4,
title = "The Effects of Interferon-γ and Bacterial Lipopolysaccharide on CD14 Expression in Human Monocytes",
abstract = "CD14 has been reported to be the lipopolysaccharide (LPS)-LPS binding protein receptor. The effects of interferon-γ (IFN-γ) on CD14 expression have not been clearly established. The purpose of this investigation was to examine the effects of IFN-γ alone and IFN-γ followed by bacterial LPS on CD14 expression. Human peripheral blood monocytes were isolated by counterflow centrifugal elutriation (CCE). Monocytes were cultured for 48 h with IFN-γ alone or for 24 h with IFN-γ followed by LPS for a second 24 h. IFN-γ alone caused a down-regulation of CD14 expression, as assessed by flow cytometry, relative to CD14 expression in untreated monocytes. In addition, CD14 expression was even more significantly down-regulated after IFN-γ pretreatment followed by either Prevotella intermedia or Salmonella typhimurium LPS. Likewise, the percentage of CD14+ monocytes decreased after IFN-γ alone and even more dramatically after IFN-γ treatment followed by either LPS. This study clearly demonstrated that IFN-γ down-regulates CD14 expression and that LPS following IFN-γ pretreatment potentiates this effect.",
author = "Payne, {Jeffrey B} and Nichols, {F. C.} and Peluso, {J. F.}",
year = "1992",
month = "8",
doi = "10.1089/jir.1992.12.307",
language = "English (US)",
volume = "12",
pages = "307--310",
journal = "Journal of Interferon Research",
issn = "0197-8357",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - The Effects of Interferon-γ and Bacterial Lipopolysaccharide on CD14 Expression in Human Monocytes

AU - Payne, Jeffrey B

AU - Nichols, F. C.

AU - Peluso, J. F.

PY - 1992/8

Y1 - 1992/8

N2 - CD14 has been reported to be the lipopolysaccharide (LPS)-LPS binding protein receptor. The effects of interferon-γ (IFN-γ) on CD14 expression have not been clearly established. The purpose of this investigation was to examine the effects of IFN-γ alone and IFN-γ followed by bacterial LPS on CD14 expression. Human peripheral blood monocytes were isolated by counterflow centrifugal elutriation (CCE). Monocytes were cultured for 48 h with IFN-γ alone or for 24 h with IFN-γ followed by LPS for a second 24 h. IFN-γ alone caused a down-regulation of CD14 expression, as assessed by flow cytometry, relative to CD14 expression in untreated monocytes. In addition, CD14 expression was even more significantly down-regulated after IFN-γ pretreatment followed by either Prevotella intermedia or Salmonella typhimurium LPS. Likewise, the percentage of CD14+ monocytes decreased after IFN-γ alone and even more dramatically after IFN-γ treatment followed by either LPS. This study clearly demonstrated that IFN-γ down-regulates CD14 expression and that LPS following IFN-γ pretreatment potentiates this effect.

AB - CD14 has been reported to be the lipopolysaccharide (LPS)-LPS binding protein receptor. The effects of interferon-γ (IFN-γ) on CD14 expression have not been clearly established. The purpose of this investigation was to examine the effects of IFN-γ alone and IFN-γ followed by bacterial LPS on CD14 expression. Human peripheral blood monocytes were isolated by counterflow centrifugal elutriation (CCE). Monocytes were cultured for 48 h with IFN-γ alone or for 24 h with IFN-γ followed by LPS for a second 24 h. IFN-γ alone caused a down-regulation of CD14 expression, as assessed by flow cytometry, relative to CD14 expression in untreated monocytes. In addition, CD14 expression was even more significantly down-regulated after IFN-γ pretreatment followed by either Prevotella intermedia or Salmonella typhimurium LPS. Likewise, the percentage of CD14+ monocytes decreased after IFN-γ alone and even more dramatically after IFN-γ treatment followed by either LPS. This study clearly demonstrated that IFN-γ down-regulates CD14 expression and that LPS following IFN-γ pretreatment potentiates this effect.

UR - http://www.scopus.com/inward/record.url?scp=0026908270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026908270&partnerID=8YFLogxK

U2 - 10.1089/jir.1992.12.307

DO - 10.1089/jir.1992.12.307

M3 - Article

C2 - 1431309

AN - SCOPUS:0026908270

VL - 12

SP - 307

EP - 310

JO - Journal of Interferon Research

JF - Journal of Interferon Research

SN - 0197-8357

IS - 4

ER -