The effects of anesthesia on the excretion of an isotonic saline load in the rat

Thomas F. Knight, Steven Sansom, Lois Hawk, Seymour J. Frankfurt, Edward J. Weinman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The ability to excrete a volume of isotonic saline equal to 10% of body weight infused over 60 min, was examined in awake rats and in rats anesthetized with 1 of the 2 agents most commonly used in renal clearance studies, Inactin or Nembutal. Rats anesthetized with Inactin excreted significantly less of the infused sodium during the period of infusion and in the 120-min post-infusion periods as compared to Nembutal-anesthetized rats or awake rats. Following saline infusion, there was a significantly greater decrease in serum protein concentration (25.5±4.7%) in rats anesthetized with Inactin, compared to that observed in the awake or Nembutal-treated rats. In a separate group of saline-infused awake rats, induction of anesthesia with Inactin resulted in a significant increase in hematocrit and a decrease in serum protein concentration. These studies suggest that Inactin anesthesia decreases the ability of the kidney to excrete a saline load and that, in studies of sodium excretion in the rat, especially if volume expansion is to be part of the experimental protocol, Nembutal rather than Inactin may be the anesthetic of choice.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalPflügers Archiv European Journal of Physiology
Volume373
Issue number2
DOIs
StatePublished - Feb 1 1978

Fingerprint

Rats
Anesthesia
Pentobarbital
Blood Proteins
Sodium
Kidney
Hematocrit
Anesthetics
thiobutabarbital
Body Weight

Keywords

  • Anesthesia
  • Sodium excretion

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

The effects of anesthesia on the excretion of an isotonic saline load in the rat. / Knight, Thomas F.; Sansom, Steven; Hawk, Lois; Frankfurt, Seymour J.; Weinman, Edward J.

In: Pflügers Archiv European Journal of Physiology, Vol. 373, No. 2, 01.02.1978, p. 139-143.

Research output: Contribution to journalArticle

Knight, Thomas F. ; Sansom, Steven ; Hawk, Lois ; Frankfurt, Seymour J. ; Weinman, Edward J. / The effects of anesthesia on the excretion of an isotonic saline load in the rat. In: Pflügers Archiv European Journal of Physiology. 1978 ; Vol. 373, No. 2. pp. 139-143.
@article{1b76a4e4f62a4d2e945501dd1c0197a2,
title = "The effects of anesthesia on the excretion of an isotonic saline load in the rat",
abstract = "The ability to excrete a volume of isotonic saline equal to 10{\%} of body weight infused over 60 min, was examined in awake rats and in rats anesthetized with 1 of the 2 agents most commonly used in renal clearance studies, Inactin or Nembutal. Rats anesthetized with Inactin excreted significantly less of the infused sodium during the period of infusion and in the 120-min post-infusion periods as compared to Nembutal-anesthetized rats or awake rats. Following saline infusion, there was a significantly greater decrease in serum protein concentration (25.5±4.7{\%}) in rats anesthetized with Inactin, compared to that observed in the awake or Nembutal-treated rats. In a separate group of saline-infused awake rats, induction of anesthesia with Inactin resulted in a significant increase in hematocrit and a decrease in serum protein concentration. These studies suggest that Inactin anesthesia decreases the ability of the kidney to excrete a saline load and that, in studies of sodium excretion in the rat, especially if volume expansion is to be part of the experimental protocol, Nembutal rather than Inactin may be the anesthetic of choice.",
keywords = "Anesthesia, Sodium excretion",
author = "Knight, {Thomas F.} and Steven Sansom and Lois Hawk and Frankfurt, {Seymour J.} and Weinman, {Edward J.}",
year = "1978",
month = "2",
day = "1",
doi = "10.1007/BF00584852",
language = "English (US)",
volume = "373",
pages = "139--143",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - The effects of anesthesia on the excretion of an isotonic saline load in the rat

AU - Knight, Thomas F.

AU - Sansom, Steven

AU - Hawk, Lois

AU - Frankfurt, Seymour J.

AU - Weinman, Edward J.

PY - 1978/2/1

Y1 - 1978/2/1

N2 - The ability to excrete a volume of isotonic saline equal to 10% of body weight infused over 60 min, was examined in awake rats and in rats anesthetized with 1 of the 2 agents most commonly used in renal clearance studies, Inactin or Nembutal. Rats anesthetized with Inactin excreted significantly less of the infused sodium during the period of infusion and in the 120-min post-infusion periods as compared to Nembutal-anesthetized rats or awake rats. Following saline infusion, there was a significantly greater decrease in serum protein concentration (25.5±4.7%) in rats anesthetized with Inactin, compared to that observed in the awake or Nembutal-treated rats. In a separate group of saline-infused awake rats, induction of anesthesia with Inactin resulted in a significant increase in hematocrit and a decrease in serum protein concentration. These studies suggest that Inactin anesthesia decreases the ability of the kidney to excrete a saline load and that, in studies of sodium excretion in the rat, especially if volume expansion is to be part of the experimental protocol, Nembutal rather than Inactin may be the anesthetic of choice.

AB - The ability to excrete a volume of isotonic saline equal to 10% of body weight infused over 60 min, was examined in awake rats and in rats anesthetized with 1 of the 2 agents most commonly used in renal clearance studies, Inactin or Nembutal. Rats anesthetized with Inactin excreted significantly less of the infused sodium during the period of infusion and in the 120-min post-infusion periods as compared to Nembutal-anesthetized rats or awake rats. Following saline infusion, there was a significantly greater decrease in serum protein concentration (25.5±4.7%) in rats anesthetized with Inactin, compared to that observed in the awake or Nembutal-treated rats. In a separate group of saline-infused awake rats, induction of anesthesia with Inactin resulted in a significant increase in hematocrit and a decrease in serum protein concentration. These studies suggest that Inactin anesthesia decreases the ability of the kidney to excrete a saline load and that, in studies of sodium excretion in the rat, especially if volume expansion is to be part of the experimental protocol, Nembutal rather than Inactin may be the anesthetic of choice.

KW - Anesthesia

KW - Sodium excretion

UR - http://www.scopus.com/inward/record.url?scp=0017804135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017804135&partnerID=8YFLogxK

U2 - 10.1007/BF00584852

DO - 10.1007/BF00584852

M3 - Article

C2 - 565042

AN - SCOPUS:0017804135

VL - 373

SP - 139

EP - 143

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 2

ER -