The effect of nucleotide substitution on DNA denaturation profiles

I. V. Razlutskii, L. S. Shlyakhtenko, Yu L. Lyubchenko

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Abstract

The melting profiles were obtained for DNA restriction fragments of approx. 1150 bp with deletion of one, five or six base pairs making them different from each other. In all cases the deletions caused a shift of one melting peak without affecting the positions of the other three peaks. The effect amounted to 0.28+ 0.03 C upon the deletion of one GC pair. The melting of DNS fragments was also studied by electrophoresis in denaturing gradient gels. The deletion of one GC pair was shown to cause an appreciable shift of the electrophoretic denaturation profile.

Original languageEnglish (US)
Pages (from-to)6665-6676
Number of pages12
JournalNucleic acids research
Volume15
Issue number16
DOIs
Publication statusPublished - Aug 25 1987

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ASJC Scopus subject areas

  • Genetics

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