The effect of Indium-111 decay on pBR322 DNA

S. K. Sahu, A. I. Kassis, G. M. Makrigiorgos, J. Baranowska-Kortylewicz, S. J. Adelstein

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Abstract

We have compared the effectiveness in causing DNA strand breaks of 111In bound to DNA or free in aqueous solution with that of γ rays. Supercoiled DNA from pBR322 plasmid labeled with [3H]thymidine was purified and mixed with 111InCl3 in the absence or presence of diethylenetriaminepentaacetic dianhydride (DTPA), a metal chelator which prevents the binding of indium to DNA. The reaction mixtures were stored at 4°C to accumulate radiation dose from the decay of 111In. The DNA was then resolved by gel electrophoresis into supercoiled, nicked circular and linear forms, representing undamaged DNA, single-strand breaks (SSBs) and double-strand breaks (DSBs), respectively. The D0 values of pBR322 DNA exposed to γ radiation from an external 137Cs source and the decay of 111In dispersed in solution (+DTPA) are 3.1 ± 0.1 and 2.8 ± 0.1 Gy, respectively. In terms of accumulated 111In disintegrations cm-3 of plasmid DNA solution, the D0 value is 15.3 (±0.7) x 1010 disintegrations in the absence of DTPA and 38.2 (±1.1) x 1010 disintegrations in its presence. Since only 14.6 ± 5% of the 111In was bound to DNA in the absence of DTPA, an effective D0 for bound 111In of 3.4 (±1.1) x 1010 disintegrations is obtained. The 11-fold (range 9- to 17-fold) increased effectiveness of this Auger electron emitter when in proximity to DNA appears to be due mainly to the higher yield of SSBs.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalRadiation Research
Volume141
Issue number2
DOIs
StatePublished - Apr 5 1995

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ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

Sahu, S. K., Kassis, A. I., Makrigiorgos, G. M., Baranowska-Kortylewicz, J., & Adelstein, S. J. (1995). The effect of Indium-111 decay on pBR322 DNA. Radiation Research, 141(2), 193-198. https://doi.org/10.2307/3579047