The effect of diabetes on expression of β1-, β2-, and β3-adrenoreceptors in rat hearts

Ü Deniz Dinçer, Keshore R Bidasee, Şahika Güner, Ayin Tay, A. Tanju Özçelikay, V. Melih Altan

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154 Citations (Scopus)

Abstract

Diabetic hearts exhibit decreased responsiveness to stimulation by β-adrenoreceptor (β-AR) agonists. This decrease in activity may be due to changes in expression and/or signaling of β-AR. Recently we showed that right atrial strips from 14-week streptozotocin (STZ)-induced diabetic rat hearts exhibit decreased responsiveness to β1-AR agonist stimulation, but not to β2-AR agonist. In the present study, we investigated the effects of long-term diabetes on the expression of cardiac β1-, β2-, and β3-ARs and looked at whether these changes could be restored with insulin treatment. Using reverse transcription-polymerase chain reaction (RT-PCR), PAGE, and Western blot analysis, we found that β1-AR mRNA and protein levels decreased by 34.9 ± 5.8 and 44.4 ± 5.8%, respectively, in 14 week-STZ-treated diabetic rat hearts when compared with age-matched controls. On the other hand, mRNA levels encoding β2- and β3-ARs increased by 72.5 ± 16.6 and 97.3 ± 26.1%, respectively. Although the latter translated into a proportional increase in β3-AR protein levels (100.0 ± 17.0%), β2-AR protein levels decreased to 82.6 ± 1.1% of control. Insulin treatment for 2 weeks, after 12 weeks of untreated diabetes, partially restored β1-AR mRNA and protein levels to 60.1 ± 8.4 and 83.2 ± 5.0%, respectively, of control. Although insulin treatment minimally attenuated the rise in mRNA levels encoding β2- and β3-ARs, the steady-state levels of these proteins returned to near control values. These data suggest that the decreased responsiveness of diabetic hearts to stimulation of β-AR agonists may be due to a decrease in β1-AR and an increase β3-AR expression.

Original languageEnglish (US)
Pages (from-to)455-461
Number of pages7
JournalDiabetes
Volume50
Issue number2
DOIs
StatePublished - Jan 1 2001

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Messenger RNA
Insulin
Streptozocin
Proteins
Reverse Transcription
Western Blotting
Polymerase Chain Reaction
Amberlite XAD-2 resin

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

The effect of diabetes on expression of β1-, β2-, and β3-adrenoreceptors in rat hearts. / Dinçer, Ü Deniz; Bidasee, Keshore R; Güner, Şahika; Tay, Ayin; Özçelikay, A. Tanju; Altan, V. Melih.

In: Diabetes, Vol. 50, No. 2, 01.01.2001, p. 455-461.

Research output: Contribution to journalArticle

Dinçer, ÜD, Bidasee, KR, Güner, Ş, Tay, A, Özçelikay, AT & Altan, VM 2001, 'The effect of diabetes on expression of β1-, β2-, and β3-adrenoreceptors in rat hearts', Diabetes, vol. 50, no. 2, pp. 455-461. https://doi.org/10.2337/diabetes.50.2.455
Dinçer, Ü Deniz ; Bidasee, Keshore R ; Güner, Şahika ; Tay, Ayin ; Özçelikay, A. Tanju ; Altan, V. Melih. / The effect of diabetes on expression of β1-, β2-, and β3-adrenoreceptors in rat hearts. In: Diabetes. 2001 ; Vol. 50, No. 2. pp. 455-461.
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