The effect of C5a and U46619 on the isolated, perfused human placental lobule: Development of a method for the online estimation of tissue fluid accumulation

Angela M. Finch, Anne E. Heron, Sean L. Tolhurst, Timothy H. Florin, Sam D. Sanderson, Stephen M. Taylor

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A method for the automatic and simultaneous determination of perfusion pressure and fluid accumulation in the isolated, perfused human placental lobule is described. We demonstrated that the inflammatory mediator, C5a, a C5a agonist analogue peptide, and the thromboxane mimetic U46619 caused increased fetal perfusion pressure and increased tissue weight when administered via the fetal arterial circulation. Occlusion of the fetal venous effluent tubing caused significantly greater increases in tissue weight than the pharmacological agents. Detectable increases in tissue weight occurred within 47 ± 3 sec (n = 21) following pressure increases caused by the pharmacological agents. In each case, the increase in tissue weight was accompanied by an increased permeability of the materno-fetal barrier, shown by the transfer of Evans blue albumin from the fetal circulation to the maternal compartment.

Original languageEnglish (US)
Pages (from-to)133-141
Number of pages9
JournalJournal of Pharmacological and Toxicological Methods
Volume34
Issue number3
DOIs
Publication statusPublished - Nov 1995

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Keywords

  • C5a
  • Perfusion pressure
  • Placenta
  • U46619
  • Weight

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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