The effect of buffer pH and molarity on the HPLC separation of alkylated purines

Carol Kolar, Terence Lawson

Research output: Contribution to journalArticle


This article describes how varying buffer pH and ionic strength affects the separation of O6-(2-hydroxypropyl)guanine, O6-methylguanine, 8-methylguanine, 7-(2-hydroxypropyl)guanine, and 7-methylguanine. The authors devised a procedure to separate C-, N-, and O-alkylguanines from one sample using two buffers: 35 mM and 125 mM ammonium phosphate (both pH 3.5). They were able to minimize the cation-exchange column degradation caused by low buffer pH.

Original languageEnglish (US)
Pages (from-to)882-885
Number of pages4
JournalLC GC: Liquid Chromatography, Gas Chromatography
Issue number10
Publication statusPublished - Oct 1 1996


ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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