The effect of buffer pH and molarity on the HPLC separation of alkylated purines

Carol Kolar, Terence Lawson

Research output: Contribution to journalArticle

Abstract

This article describes how varying buffer pH and ionic strength affects the separation of O6-(2-hydroxypropyl)guanine, O6-methylguanine, 8-methylguanine, 7-(2-hydroxypropyl)guanine, and 7-methylguanine. The authors devised a procedure to separate C-, N-, and O-alkylguanines from one sample using two buffers: 35 mM and 125 mM ammonium phosphate (both pH 3.5). They were able to minimize the cation-exchange column degradation caused by low buffer pH.

Original languageEnglish (US)
Pages (from-to)882-885
Number of pages4
JournalLC GC: Liquid Chromatography, Gas Chromatography
Volume14
Issue number10
StatePublished - Oct 1 1996

Fingerprint

Purines
Ionic strength
Ion exchange
Buffers
Phosphates
Positive ions
Degradation
Guanine
Cations

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

Cite this

The effect of buffer pH and molarity on the HPLC separation of alkylated purines. / Kolar, Carol; Lawson, Terence.

In: LC GC: Liquid Chromatography, Gas Chromatography, Vol. 14, No. 10, 01.10.1996, p. 882-885.

Research output: Contribution to journalArticle

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