This article describes how varying buffer pH and ionic strength affects the separation of O6-(2-hydroxypropyl)guanine, O6-methylguanine, 8-methylguanine, 7-(2-hydroxypropyl)guanine, and 7-methylguanine. The authors devised a procedure to separate C-, N-, and O-alkylguanines from one sample using two buffers: 35 mM and 125 mM ammonium phosphate (both pH 3.5). They were able to minimize the cation-exchange column degradation caused by low buffer pH.
|Original language||English (US)|
|Number of pages||4|
|Journal||LC GC: Liquid Chromatography, Gas Chromatography|
|Publication status||Published - Oct 1 1996|
ASJC Scopus subject areas
- Analytical Chemistry
- Filtration and Separation