The distribution of guanine-cytosine pairs in adenovirus DNAs

T. I. Tikchonenko, A. G. Dubichev, Y. L. Lyubchenko, N. P. Kvitko, N. M. Chaplygina, T. I. Kalinina, R. S. Dreizin, B. S. Naroditsky

Research output: Contribution to journalArticle

Abstract

The distribution of guanine-cytosine (GC) pairs in the DNA of the highly oncogenic simian adenovirus type 7 (SA7) and the non-oncogenic human adenovirus type 6 (Ad6) has been studied by thermal denaturation and CsCl density-gradient centrifugation. The differential of the DNA thermal denaturation curves shows the presence of pronounced peaks which indicates uneven distribution of GC pairs along the DNA chains and the presence of regions with GC content from 30 to 74% in SA7 DNA and from 40 to 68% in Ad6 DNA. The DNA restriction fragments obtained by treatment with EcoRI, RamHI, SalI, BglII and HindIII were subjected to CsCl density-gradient centrifugation. GC content of the fragments ranged from 45 to 70% for SA7 DNA and from 43 to 61% for Ad6 DNA. The GC content of the extreme left-hand fragments, where the transforming gene(s) is located, was higher than the average for SA7 DNA and lower than the average for Ad6 DNA. The most GC-rich regions were localized in the centre of the genome. The GC content of the right-hand part of both viral genomes was lower than the average.

Original languageEnglish (US)
Pages (from-to)425-429
Number of pages5
JournalUnknown Journal
Volume54
Issue number2
DOIs
StatePublished - Jan 1 1981

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Cytosine
Guanine
Adenoviridae
Simian Adenoviruses
DNA
Base Composition
Density Gradient Centrifugation
Hand
Hot Temperature
Nucleic Acid Denaturation
Human Adenoviruses
Viral Genome
Oncogenes
Genome

ASJC Scopus subject areas

  • Virology

Cite this

Tikchonenko, T. I., Dubichev, A. G., Lyubchenko, Y. L., Kvitko, N. P., Chaplygina, N. M., Kalinina, T. I., ... Naroditsky, B. S. (1981). The distribution of guanine-cytosine pairs in adenovirus DNAs. Unknown Journal, 54(2), 425-429. https://doi.org/10.1099/0022-1317-54-2-425

The distribution of guanine-cytosine pairs in adenovirus DNAs. / Tikchonenko, T. I.; Dubichev, A. G.; Lyubchenko, Y. L.; Kvitko, N. P.; Chaplygina, N. M.; Kalinina, T. I.; Dreizin, R. S.; Naroditsky, B. S.

In: Unknown Journal, Vol. 54, No. 2, 01.01.1981, p. 425-429.

Research output: Contribution to journalArticle

Tikchonenko, TI, Dubichev, AG, Lyubchenko, YL, Kvitko, NP, Chaplygina, NM, Kalinina, TI, Dreizin, RS & Naroditsky, BS 1981, 'The distribution of guanine-cytosine pairs in adenovirus DNAs', Unknown Journal, vol. 54, no. 2, pp. 425-429. https://doi.org/10.1099/0022-1317-54-2-425
Tikchonenko TI, Dubichev AG, Lyubchenko YL, Kvitko NP, Chaplygina NM, Kalinina TI et al. The distribution of guanine-cytosine pairs in adenovirus DNAs. Unknown Journal. 1981 Jan 1;54(2):425-429. https://doi.org/10.1099/0022-1317-54-2-425
Tikchonenko, T. I. ; Dubichev, A. G. ; Lyubchenko, Y. L. ; Kvitko, N. P. ; Chaplygina, N. M. ; Kalinina, T. I. ; Dreizin, R. S. ; Naroditsky, B. S. / The distribution of guanine-cytosine pairs in adenovirus DNAs. In: Unknown Journal. 1981 ; Vol. 54, No. 2. pp. 425-429.
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