The distorted α2-adrenoceptor genotype distribution in F2 populations of dahl salt sensitive and resistant rats cross

Lu Sun, Melanie Chun, Shari McArdle, William J Kimberling, William Pettinger

Research output: Contribution to journalArticle

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Abstract

To examine the role of the α2-adrenoceptor gene in the development of genetic hypertensive rats, we tested Dahl salt sensitive (S) and resistant rats (R) for the presence of a restriction fragment length polymorphism (RFLP) in that gene. An RFLP was found between the S and R rats with a human kidney cDNA α2-adrenoceptor probe (α2-C4) and Msp I restriction endonuclease. The α2-C4 probe detected two alleles, S and R, of 3.0 and 2.8 kb in size. The two strains of rats were each homozygous for their corresponding allele. The inheritance of the alleles was investigated by crossbreeding S and R rats and subsequent brother/sister mating of F1 rats. Two hundred and fifteen F2 rats were produced by breeding 14 pairs of F1 rats. An atypical distribution of α2-adrenoceptor genotypes was observed. We found a reduced number of the SS genotype in both males and females of the F2 generation. To investigate the mechanism of this distorted α2-adnenoceptor genotype distribution in the F2 rats of S and R cross, we backrossed the F1 rats to their S and R parents. The litter size and gender distribution were counted for each breeding colony. Analysis by chi-square test showed that there was no sex difference among the backcrosses. Also, there was no significant decrease in litter size. This excludes the possibilities of fetal demise of S homozygotes and intrauterine selection. Therefore the deficiency of SS genotype may be due to gene recombination or may not be due to the α2-adrenoceptor gene itself, but to the effect of other genes closely linked to the α2-adrenoceptor.

Original languageEnglish (US)
JournalLife Sciences
Volume53
Issue number4
DOIs
StatePublished - Jan 1 1993

Fingerprint

Inbred Dahl Rats
Adrenergic Receptors
Rats
Salts
Genotype
Population
Genes
Litter Size
Alleles
Restriction Fragment Length Polymorphisms
Polymorphism
Breeding
Genetic Hybridization
Fetal Death
DNA Restriction Enzymes
Homozygote
Chi-Square Distribution
Sex Characteristics
Genetic Recombination
Complementary DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

The distorted α2-adrenoceptor genotype distribution in F2 populations of dahl salt sensitive and resistant rats cross. / Sun, Lu; Chun, Melanie; McArdle, Shari; Kimberling, William J; Pettinger, William.

In: Life Sciences, Vol. 53, No. 4, 01.01.1993.

Research output: Contribution to journalArticle

Sun, Lu ; Chun, Melanie ; McArdle, Shari ; Kimberling, William J ; Pettinger, William. / The distorted α2-adrenoceptor genotype distribution in F2 populations of dahl salt sensitive and resistant rats cross. In: Life Sciences. 1993 ; Vol. 53, No. 4.
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