The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages

Jingwei Xie, Stephanie M. Willerth, Xiaoran Li, Matthew R. Macewan, Allison Rader, Shelly E. Sakiyama-Elbert, Younan Xia

Research output: Contribution to journalArticle

313 Citations (Scopus)

Abstract

Due to advances in stem cell biology, embryonic stem (ES) cells can be induced to differentiate into a particular mature cell lineage when cultured as embryoid bodies. Although transplantation of ES cells-derived neural progenitor cells has been demonstrated with some success for either spinal cord injury repair in small animal model, control of ES cell differentiation into complex, viable, higher ordered tissues is still challenging. Mouse ES cells have been induced to become neural progenitors by adding retinoic acid to embryoid body cultures for 4 days. In this study, we examine the use of electrospun biodegradable polymers as scaffolds not only for enhancing the differentiation of mouse ES cells into neural lineages but also for promoting and guiding the neurite outgrowth. A combination of electrospun fiber scaffolds and ES cells-derived neural progenitor cells could lead to the development of a better strategy for nerve injury repair.

Original languageEnglish (US)
Pages (from-to)354-362
Number of pages9
JournalBiomaterials
Volume30
Issue number3
DOIs
StatePublished - Jan 1 2009

Fingerprint

Nanofibers
Embryonic Stem Cells
Stem cells
Embryoid Bodies
Stem Cells
Spinal Cord Regeneration
Scaffolds
Cell Lineage
Tretinoin
Spinal Cord Injuries
Repair
Cell Biology
Cell Differentiation
Polymers
Cytology
Animal Models
Transplantation
Biodegradable polymers
Wounds and Injuries
Animals

Keywords

  • Differentiation
  • Electrospinning
  • Embryonic stem cell
  • Nanofibers
  • Nerve repair
  • Poly(ε-caprolactone)

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Xie, J., Willerth, S. M., Li, X., Macewan, M. R., Rader, A., Sakiyama-Elbert, S. E., & Xia, Y. (2009). The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages. Biomaterials, 30(3), 354-362. https://doi.org/10.1016/j.biomaterials.2008.09.046

The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages. / Xie, Jingwei; Willerth, Stephanie M.; Li, Xiaoran; Macewan, Matthew R.; Rader, Allison; Sakiyama-Elbert, Shelly E.; Xia, Younan.

In: Biomaterials, Vol. 30, No. 3, 01.01.2009, p. 354-362.

Research output: Contribution to journalArticle

Xie, J, Willerth, SM, Li, X, Macewan, MR, Rader, A, Sakiyama-Elbert, SE & Xia, Y 2009, 'The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages', Biomaterials, vol. 30, no. 3, pp. 354-362. https://doi.org/10.1016/j.biomaterials.2008.09.046
Xie, Jingwei ; Willerth, Stephanie M. ; Li, Xiaoran ; Macewan, Matthew R. ; Rader, Allison ; Sakiyama-Elbert, Shelly E. ; Xia, Younan. / The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages. In: Biomaterials. 2009 ; Vol. 30, No. 3. pp. 354-362.
@article{3e3f1e1afcdf435e83c560bf2086d6f2,
title = "The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages",
abstract = "Due to advances in stem cell biology, embryonic stem (ES) cells can be induced to differentiate into a particular mature cell lineage when cultured as embryoid bodies. Although transplantation of ES cells-derived neural progenitor cells has been demonstrated with some success for either spinal cord injury repair in small animal model, control of ES cell differentiation into complex, viable, higher ordered tissues is still challenging. Mouse ES cells have been induced to become neural progenitors by adding retinoic acid to embryoid body cultures for 4 days. In this study, we examine the use of electrospun biodegradable polymers as scaffolds not only for enhancing the differentiation of mouse ES cells into neural lineages but also for promoting and guiding the neurite outgrowth. A combination of electrospun fiber scaffolds and ES cells-derived neural progenitor cells could lead to the development of a better strategy for nerve injury repair.",
keywords = "Differentiation, Electrospinning, Embryonic stem cell, Nanofibers, Nerve repair, Poly(ε-caprolactone)",
author = "Jingwei Xie and Willerth, {Stephanie M.} and Xiaoran Li and Macewan, {Matthew R.} and Allison Rader and Sakiyama-Elbert, {Shelly E.} and Younan Xia",
year = "2009",
month = "1",
day = "1",
doi = "10.1016/j.biomaterials.2008.09.046",
language = "English (US)",
volume = "30",
pages = "354--362",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages

AU - Xie, Jingwei

AU - Willerth, Stephanie M.

AU - Li, Xiaoran

AU - Macewan, Matthew R.

AU - Rader, Allison

AU - Sakiyama-Elbert, Shelly E.

AU - Xia, Younan

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Due to advances in stem cell biology, embryonic stem (ES) cells can be induced to differentiate into a particular mature cell lineage when cultured as embryoid bodies. Although transplantation of ES cells-derived neural progenitor cells has been demonstrated with some success for either spinal cord injury repair in small animal model, control of ES cell differentiation into complex, viable, higher ordered tissues is still challenging. Mouse ES cells have been induced to become neural progenitors by adding retinoic acid to embryoid body cultures for 4 days. In this study, we examine the use of electrospun biodegradable polymers as scaffolds not only for enhancing the differentiation of mouse ES cells into neural lineages but also for promoting and guiding the neurite outgrowth. A combination of electrospun fiber scaffolds and ES cells-derived neural progenitor cells could lead to the development of a better strategy for nerve injury repair.

AB - Due to advances in stem cell biology, embryonic stem (ES) cells can be induced to differentiate into a particular mature cell lineage when cultured as embryoid bodies. Although transplantation of ES cells-derived neural progenitor cells has been demonstrated with some success for either spinal cord injury repair in small animal model, control of ES cell differentiation into complex, viable, higher ordered tissues is still challenging. Mouse ES cells have been induced to become neural progenitors by adding retinoic acid to embryoid body cultures for 4 days. In this study, we examine the use of electrospun biodegradable polymers as scaffolds not only for enhancing the differentiation of mouse ES cells into neural lineages but also for promoting and guiding the neurite outgrowth. A combination of electrospun fiber scaffolds and ES cells-derived neural progenitor cells could lead to the development of a better strategy for nerve injury repair.

KW - Differentiation

KW - Electrospinning

KW - Embryonic stem cell

KW - Nanofibers

KW - Nerve repair

KW - Poly(ε-caprolactone)

UR - http://www.scopus.com/inward/record.url?scp=55249093369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=55249093369&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2008.09.046

DO - 10.1016/j.biomaterials.2008.09.046

M3 - Article

VL - 30

SP - 354

EP - 362

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 3

ER -