The dehydratase ADT3 affects ROS homeostasis and cotyledon development

Alessia Para, Durre Shahwar Muhammad, Danielle A. Orozco-Nunnelly, Ramis Memishi, Sophie Alvarez, Michael J. Naldrett, Katherine M. Warpeha

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

During the transition from seed to seedling, emerging embryos strategically balance available resources between building up defenses against environmental threats and initiating the developmental program that promotes the switch to autotrophy. We present evidence of a critical role for the phenylalanine (Phe) biosynthetic activity of AROGENATE DEHYDRATASE3 (ADT3) in coordinating reactive oxygen species (ROS) homeostasis and cotyledon development in etiolated Arabidopsis (Arabidopsis thaliana) seedlings. We show that ADT3 is expressed in the cotyledon and shoot apical meristem, mainly in the cytosol, and that the epidermis of adt3 cotyledons contains higher levels of ROS. Genome-wide proteomics of the adt3 mutant revealed a general down-regulation of plastidic proteins and ROS-scavenging enzymes, corroborating the hypothesis that the ADT3 supply of Phe is required to control ROS concentration and distribution to protect cellular components. In addition, loss of ADT3 disrupts cotyledon epidermal patterning by affecting the number and expansion of pavement cells and stomata cell fate specification; we also observed severe alterations in mesophyll cells, which lack oil bodies and normal plastids. Interestingly, up-regulation of the pathway leading to cuticle production is accompanied by an abnormal cuticle structure and/or deposition in the adt3 mutant. Such impairment results in an increase in cell permeability and provides a link to understand the cell defects in the adt3 cotyledon epidermis. We suggest an additional role of Phe in supplying nutrients to the young seedling.

Original languageEnglish (US)
Pages (from-to)1045-1060
Number of pages16
JournalPlant physiology
Volume172
Issue number2
DOIs
StatePublished - Oct 2016

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Hydro-Lyases
Cotyledon
cotyledons
reactive oxygen species
Reactive Oxygen Species
homeostasis
Homeostasis
Phenylalanine
Seedlings
phenylalanine
epidermis (plant)
Arabidopsis
Epidermis
cells
seedlings
Autotrophic Processes
Mesophyll Cells
mutants
Plastids
Meristem

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Para, A., Muhammad, D. S., Orozco-Nunnelly, D. A., Memishi, R., Alvarez, S., Naldrett, M. J., & Warpeha, K. M. (2016). The dehydratase ADT3 affects ROS homeostasis and cotyledon development. Plant physiology, 172(2), 1045-1060. https://doi.org/10.1104/pp.16.00464

The dehydratase ADT3 affects ROS homeostasis and cotyledon development. / Para, Alessia; Muhammad, Durre Shahwar; Orozco-Nunnelly, Danielle A.; Memishi, Ramis; Alvarez, Sophie; Naldrett, Michael J.; Warpeha, Katherine M.

In: Plant physiology, Vol. 172, No. 2, 10.2016, p. 1045-1060.

Research output: Contribution to journalArticle

Para, A, Muhammad, DS, Orozco-Nunnelly, DA, Memishi, R, Alvarez, S, Naldrett, MJ & Warpeha, KM 2016, 'The dehydratase ADT3 affects ROS homeostasis and cotyledon development', Plant physiology, vol. 172, no. 2, pp. 1045-1060. https://doi.org/10.1104/pp.16.00464
Para, Alessia ; Muhammad, Durre Shahwar ; Orozco-Nunnelly, Danielle A. ; Memishi, Ramis ; Alvarez, Sophie ; Naldrett, Michael J. ; Warpeha, Katherine M. / The dehydratase ADT3 affects ROS homeostasis and cotyledon development. In: Plant physiology. 2016 ; Vol. 172, No. 2. pp. 1045-1060.
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