The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus

Tatiana Rochat, Chantal Bohn, Claire Morvan, Thao Nguyen Le Lam, Fareha Razvi, Adrien Pain, Claire Toffano-Nioche, Prishila Ponien, Annick Jacq, Eric Jacquet, Paul D Fey, Daniel Gautheret, Philippe Bouloc

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

RsaE is a regulatory RNA highly conserved amongst Firmicutes that lowers the amount of mRNAs associated with the TCA cycle and folate metabolism. A search for new RsaE targets in Staphylococcus aureus revealed that in addition to previously described substrates, RsaE down-regulates several genes associated with arginine catabolism. In particular, RsaE targets the arginase rocF mRNA via direct interactions involving G-rich motifs. Two duplicated C-rich motifs of RsaE can independently downregulate rocF expression. The faster growth rate of rsaE compared to its parental strain in media containing amino acids as sole carbon source points to an underlying role for RsaE in amino acid catabolism. Collectively, the data support a model in which RsaE acts as a global regulator of functions associated with metabolic adaptation.

Original languageEnglish (US)
Pages (from-to)8803-8816
Number of pages14
JournalNucleic acids research
Volume46
Issue number17
DOIs
StatePublished - Jan 1 2018

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Arginine
Staphylococcus aureus
Down-Regulation
RNA
Amino Acids
Arginase
Messenger RNA
Folic Acid
Carbon
Growth
Genes
Firmicutes

ASJC Scopus subject areas

  • Genetics

Cite this

Rochat, T., Bohn, C., Morvan, C., Le Lam, T. N., Razvi, F., Pain, A., ... Bouloc, P. (2018). The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus. Nucleic acids research, 46(17), 8803-8816. https://doi.org/10.1093/nar/gky584

The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus. / Rochat, Tatiana; Bohn, Chantal; Morvan, Claire; Le Lam, Thao Nguyen; Razvi, Fareha; Pain, Adrien; Toffano-Nioche, Claire; Ponien, Prishila; Jacq, Annick; Jacquet, Eric; Fey, Paul D; Gautheret, Daniel; Bouloc, Philippe.

In: Nucleic acids research, Vol. 46, No. 17, 01.01.2018, p. 8803-8816.

Research output: Contribution to journalArticle

Rochat, T, Bohn, C, Morvan, C, Le Lam, TN, Razvi, F, Pain, A, Toffano-Nioche, C, Ponien, P, Jacq, A, Jacquet, E, Fey, PD, Gautheret, D & Bouloc, P 2018, 'The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus', Nucleic acids research, vol. 46, no. 17, pp. 8803-8816. https://doi.org/10.1093/nar/gky584
Rochat, Tatiana ; Bohn, Chantal ; Morvan, Claire ; Le Lam, Thao Nguyen ; Razvi, Fareha ; Pain, Adrien ; Toffano-Nioche, Claire ; Ponien, Prishila ; Jacq, Annick ; Jacquet, Eric ; Fey, Paul D ; Gautheret, Daniel ; Bouloc, Philippe. / The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus. In: Nucleic acids research. 2018 ; Vol. 46, No. 17. pp. 8803-8816.
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