The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000

C. Robin Buell, Vinita Joardar, Magdalen Lindeberg, Jeremy Selengut, Ian T. Paulsen, Michelle L. Gwinn, Robert J. Dodson, Robert T. Deboy, A. Scott Durkin, James F. Kolonay, Ramana Madupu, Sean Daugherty, Lauren Brinkac, Maureen J. Beanan, Daniel H. Haft, William C. Nelson, Tanja Davidsen, Nikhat Zafar, Liwei Zhou, Jia Liu & 24 others Qiaoping Yuan, Hoda Khouri, Nadia Fedorova, Bao Tran, Daniel Russell, Kristi Berry, Teresa Utterback, Susan E. Van Aken, Tamara V. Feldblyum, Mark D'Ascenzo, Wen Ling Deng, Adela R. Ramos, James R Alfano, Samuel Cartinhour, Arun K. Chatterjee, Terrence P. Delaney, Sondra G. Lazarowitz, Gregory B. Martin, David J. Schneider, Xiaoyan Tang, Carol L. Bender, Owen White, Claire M. Fraser, Alan Collmer

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550 Citations (Scopus)

Abstract

We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome (6.5 megabases) contains a circular chromosome and two plasmids, which collectively encode 5,763 ORFs. We identified 298 established and putative virulence genes, including several clusters of genes encoding 31 confirmed and 19 predicted type III secretion system effector proteins. Many of the virulence genes were members of paralogous families and also were proximal to mobile elements, which collectively comprise 7% of the DC3000 genome. The bacterium possesses a large repertoire of transporters for the acquisition of nutrients, particularly sugars, as well as genes implicated in attachment to plant surfaces. Over 12% of the genes are dedicated to regulation, which may reflect the need for rapid adaptation to the diverse environments encountered during epiphytic growth and pathogenesis. Comparative analyses confirmed a high degree of similarity with two sequenced pseudomonads, Pseudomonas putida and Pseudomonas aeruginosa, yet revealed 1,159 genes unique to DC3000, of which 811 lack a known function.

Original languageEnglish (US)
Pages (from-to)10181-10186
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number18
DOIs
StatePublished - Sep 2 2003

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Pseudomonas syringae
Lycopersicon esculentum
Arabidopsis
Genome
Genes
Virulence
Pseudomonas putida
Multigene Family
Pseudomonas aeruginosa
Open Reading Frames
Plasmids
Chromosomes
Bacteria
Food
Growth
Proteins

ASJC Scopus subject areas

  • General

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The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000. / Buell, C. Robin; Joardar, Vinita; Lindeberg, Magdalen; Selengut, Jeremy; Paulsen, Ian T.; Gwinn, Michelle L.; Dodson, Robert J.; Deboy, Robert T.; Durkin, A. Scott; Kolonay, James F.; Madupu, Ramana; Daugherty, Sean; Brinkac, Lauren; Beanan, Maureen J.; Haft, Daniel H.; Nelson, William C.; Davidsen, Tanja; Zafar, Nikhat; Zhou, Liwei; Liu, Jia; Yuan, Qiaoping; Khouri, Hoda; Fedorova, Nadia; Tran, Bao; Russell, Daniel; Berry, Kristi; Utterback, Teresa; Van Aken, Susan E.; Feldblyum, Tamara V.; D'Ascenzo, Mark; Deng, Wen Ling; Ramos, Adela R.; Alfano, James R; Cartinhour, Samuel; Chatterjee, Arun K.; Delaney, Terrence P.; Lazarowitz, Sondra G.; Martin, Gregory B.; Schneider, David J.; Tang, Xiaoyan; Bender, Carol L.; White, Owen; Fraser, Claire M.; Collmer, Alan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 18, 02.09.2003, p. 10181-10186.

Research output: Contribution to journalArticle

Buell, CR, Joardar, V, Lindeberg, M, Selengut, J, Paulsen, IT, Gwinn, ML, Dodson, RJ, Deboy, RT, Durkin, AS, Kolonay, JF, Madupu, R, Daugherty, S, Brinkac, L, Beanan, MJ, Haft, DH, Nelson, WC, Davidsen, T, Zafar, N, Zhou, L, Liu, J, Yuan, Q, Khouri, H, Fedorova, N, Tran, B, Russell, D, Berry, K, Utterback, T, Van Aken, SE, Feldblyum, TV, D'Ascenzo, M, Deng, WL, Ramos, AR, Alfano, JR, Cartinhour, S, Chatterjee, AK, Delaney, TP, Lazarowitz, SG, Martin, GB, Schneider, DJ, Tang, X, Bender, CL, White, O, Fraser, CM & Collmer, A 2003, 'The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 18, pp. 10181-10186. https://doi.org/10.1073/pnas.1731982100
Buell, C. Robin ; Joardar, Vinita ; Lindeberg, Magdalen ; Selengut, Jeremy ; Paulsen, Ian T. ; Gwinn, Michelle L. ; Dodson, Robert J. ; Deboy, Robert T. ; Durkin, A. Scott ; Kolonay, James F. ; Madupu, Ramana ; Daugherty, Sean ; Brinkac, Lauren ; Beanan, Maureen J. ; Haft, Daniel H. ; Nelson, William C. ; Davidsen, Tanja ; Zafar, Nikhat ; Zhou, Liwei ; Liu, Jia ; Yuan, Qiaoping ; Khouri, Hoda ; Fedorova, Nadia ; Tran, Bao ; Russell, Daniel ; Berry, Kristi ; Utterback, Teresa ; Van Aken, Susan E. ; Feldblyum, Tamara V. ; D'Ascenzo, Mark ; Deng, Wen Ling ; Ramos, Adela R. ; Alfano, James R ; Cartinhour, Samuel ; Chatterjee, Arun K. ; Delaney, Terrence P. ; Lazarowitz, Sondra G. ; Martin, Gregory B. ; Schneider, David J. ; Tang, Xiaoyan ; Bender, Carol L. ; White, Owen ; Fraser, Claire M. ; Collmer, Alan. / The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 18. pp. 10181-10186.
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abstract = "We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome (6.5 megabases) contains a circular chromosome and two plasmids, which collectively encode 5,763 ORFs. We identified 298 established and putative virulence genes, including several clusters of genes encoding 31 confirmed and 19 predicted type III secretion system effector proteins. Many of the virulence genes were members of paralogous families and also were proximal to mobile elements, which collectively comprise 7{\%} of the DC3000 genome. The bacterium possesses a large repertoire of transporters for the acquisition of nutrients, particularly sugars, as well as genes implicated in attachment to plant surfaces. Over 12{\%} of the genes are dedicated to regulation, which may reflect the need for rapid adaptation to the diverse environments encountered during epiphytic growth and pathogenesis. Comparative analyses confirmed a high degree of similarity with two sequenced pseudomonads, Pseudomonas putida and Pseudomonas aeruginosa, yet revealed 1,159 genes unique to DC3000, of which 811 lack a known function.",
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T1 - The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000

AU - Buell, C. Robin

AU - Joardar, Vinita

AU - Lindeberg, Magdalen

AU - Selengut, Jeremy

AU - Paulsen, Ian T.

AU - Gwinn, Michelle L.

AU - Dodson, Robert J.

AU - Deboy, Robert T.

AU - Durkin, A. Scott

AU - Kolonay, James F.

AU - Madupu, Ramana

AU - Daugherty, Sean

AU - Brinkac, Lauren

AU - Beanan, Maureen J.

AU - Haft, Daniel H.

AU - Nelson, William C.

AU - Davidsen, Tanja

AU - Zafar, Nikhat

AU - Zhou, Liwei

AU - Liu, Jia

AU - Yuan, Qiaoping

AU - Khouri, Hoda

AU - Fedorova, Nadia

AU - Tran, Bao

AU - Russell, Daniel

AU - Berry, Kristi

AU - Utterback, Teresa

AU - Van Aken, Susan E.

AU - Feldblyum, Tamara V.

AU - D'Ascenzo, Mark

AU - Deng, Wen Ling

AU - Ramos, Adela R.

AU - Alfano, James R

AU - Cartinhour, Samuel

AU - Chatterjee, Arun K.

AU - Delaney, Terrence P.

AU - Lazarowitz, Sondra G.

AU - Martin, Gregory B.

AU - Schneider, David J.

AU - Tang, Xiaoyan

AU - Bender, Carol L.

AU - White, Owen

AU - Fraser, Claire M.

AU - Collmer, Alan

PY - 2003/9/2

Y1 - 2003/9/2

N2 - We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome (6.5 megabases) contains a circular chromosome and two plasmids, which collectively encode 5,763 ORFs. We identified 298 established and putative virulence genes, including several clusters of genes encoding 31 confirmed and 19 predicted type III secretion system effector proteins. Many of the virulence genes were members of paralogous families and also were proximal to mobile elements, which collectively comprise 7% of the DC3000 genome. The bacterium possesses a large repertoire of transporters for the acquisition of nutrients, particularly sugars, as well as genes implicated in attachment to plant surfaces. Over 12% of the genes are dedicated to regulation, which may reflect the need for rapid adaptation to the diverse environments encountered during epiphytic growth and pathogenesis. Comparative analyses confirmed a high degree of similarity with two sequenced pseudomonads, Pseudomonas putida and Pseudomonas aeruginosa, yet revealed 1,159 genes unique to DC3000, of which 811 lack a known function.

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