The competitive binding of STAT3 and NF-κB on an overlapping DNA binding site

Zhixin Zhang, Gerald M. Fuller

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Interleukin-1 (IL-1) and interleukin-6 (IL-6), two early-response cytokines expressed during an acute inflammatory reaction, regulate the expression of several acute phase proteins (APP) in the liver. IL-1 relays its signal to specific genes via NF-κB, whereas IL-6 sends its signal to the nucleus via STATα and STAT3. Interestingly, overlapping binding sites for STAT3 and NF-κB can be found on promoters of several APP genes. We show here that both STAT3 and NF-κB are active during inflammation and are capable of binding to a STAT3/NF-κB overlapping DNA motif derived from the α2-macroglobulin gene promoter. In vitro binding assays demonstrated that NF-κB competes with STAT3 binding on this probe. Our results suggest that these transcription factors regulate each others' function through competition for overlapping DNA binding sites.

Original languageEnglish (US)
Pages (from-to)90-94
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume237
Issue number1
DOIs
Publication statusPublished - Aug 8 1997

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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