The coat protein of turnip crinkle virus suppresses posttranscriptional gene silencing at an early initiation step

Feng Qu, Tao Ren, T. Jack Morris

Research output: Contribution to journalArticle

227 Citations (Scopus)

Abstract

Posttranscriptional gene silencing (PTGS), or RNA silencing, is a sequence-specific RNA degradation process that targets foreign RNA, including viral and transposon RNA for destruction, Several RNA plant viruses have been shown to encode suppressors of PTGS in order to survive this host defense, We report here that the coat protein (CP) of Turnip crinkle virus (TCV) strongly suppresses PTGS. The Agrobacterium infiltration system was used to demonstrate that TCV CP suppressed the local PTGS as strongly as several previously reported virus-coded suppressors and that the action of TCV CP eliminated the small interfering RNAs associated with PTGS. We have also shown that the TCV CP must be present at the time of silencing initiation to be an effective suppressor. TCV CP was able to suppress PTGS induced by sense, antisense, and double-stranded RNAs, and it prevented systemic silencing, These data suggest that TCV CP functions to suppress RNA silencing at an early initiation step, likely by interfering the function of the Dicer-like RNase in plants.

Original languageEnglish (US)
Pages (from-to)511-522
Number of pages12
JournalJournal of virology
Volume77
Issue number1
DOIs
StatePublished - Jan 1 2003

Fingerprint

Carmovirus
Turnip crinkle virus
Capsid Proteins
RNA Interference
coat proteins
RNA interference
RNA
Suppressor Genes
Plant Viruses
Agrobacterium
Double-Stranded RNA
RNA Viruses
plant viruses
double-stranded RNA
RNA Stability
Viral RNA
Ribonucleases
small interfering RNA
transposons
Small Interfering RNA

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

The coat protein of turnip crinkle virus suppresses posttranscriptional gene silencing at an early initiation step. / Qu, Feng; Ren, Tao; Morris, T. Jack.

In: Journal of virology, Vol. 77, No. 1, 01.01.2003, p. 511-522.

Research output: Contribution to journalArticle

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