The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein

P. J. Brixey, C. Guda, H. Daniell

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The T7 promoter is commonly used in E. coli expression studies but has several disadvantages due to induction with isopropylthio-β-D-galactoside (IPTG) including the prohibitive cost of IPTG, reduction in cell growth, and the poisonous nature of IPTG prohibiting its use in pharmaceutical products. The chloroplast psbA promoter produces an 18.5 fold increase in expression in E. coli compared to the T7 promoter. Also, the psbA promoter does not have the disadvantages of induction with IPTG.

Original languageEnglish (US)
Pages (from-to)395-400
Number of pages6
JournalBiotechnology Letters
Volume19
Issue number4
DOIs
StatePublished - Jan 1 1997

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Galactosides
Chloroplasts
Escherichia coli
Proteins
Cell growth
Drug products
Costs and Cost Analysis
Growth
Pharmaceutical Preparations
Costs

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein. / Brixey, P. J.; Guda, C.; Daniell, H.

In: Biotechnology Letters, Vol. 19, No. 4, 01.01.1997, p. 395-400.

Research output: Contribution to journalArticle

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