The cellular and molecular basis of health benefits of grape seed proanthocyanidin extract

Shantaram S Joshi, C. A. Kuszynski, D. Bagchi

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Red grape seed extract containing proanthocyanidins and other antioxidants are being used as nutritional supplements by many health conscious individuals. The beneficial effects of grape seed proanthocyanidins (GSPE) have been reported, however, little is known about their mechanism(s) of action. One of the beneficial effects of GSPE is chemoprevention if cellular damage. The precise mechanism by which GSPE mediates, chemoprevention is not yet understood. This report addresses this issue. We investigated the mechanisms of actions of GSPE, which ameliorates chemotherapy-induced toxic effects of Idarubicin (Ida) and 4,-hydroxyperoxycyclophosphamide (4-HC) in normal human Chang liver cells. Exposure to GSPE resulted in a significant reduction in apoptosis in response to the cytotoxicity of chemotherapeutic agents. RT-PCR analysis showed a significant increase in the anti-apoptotic gene Bcl-2 and a decrease in the cell cycle associated and proapoptotic genes, c-myc and p53 in cells treated with GSPE. These results suggest that some of the chemopreventive effects of GSPE are mediated by upregulating Bcl-2 and down regulating c-myc and p53 genes.

Original languageEnglish (US)
Pages (from-to)187-200
Number of pages14
JournalCurrent Pharmaceutical Biotechnology
Volume2
Issue number2
DOIs
StatePublished - Jun 5 2001

Fingerprint

Grape Seed Extract
myc Genes
Chemoprevention
Insurance Benefits
Idarubicin
bcl-2 Genes
Proanthocyanidins
Poisons
p53 Genes
Cell Cycle
Antioxidants
Apoptosis
Drug Therapy
Polymerase Chain Reaction
Liver
Health
Grape Seed Proanthocyanidins

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Molecular Medicine
  • Biotechnology

Cite this

The cellular and molecular basis of health benefits of grape seed proanthocyanidin extract. / Joshi, Shantaram S; Kuszynski, C. A.; Bagchi, D.

In: Current Pharmaceutical Biotechnology, Vol. 2, No. 2, 05.06.2001, p. 187-200.

Research output: Contribution to journalArticle

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