The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: Genes are embedded in the LPS region

Yuansha Chen, Peter Bystricky, Jacob Adeyeye, Pinaki Panigrahi, Afsar Ali, Judith A. Johnson, C. A. Bush, J. G. Morris, O. C. Stine

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background. In V. cholerae, the biogenesis of capsule polysaccharide is poorly understood. The elucidation of capsule structure and biogenesis is critical to understanding the evolution of surface polysaccharide and the internal relationship between the capsule and LPS in this species. V. cholerae serogroup O31 NRT36S, a human pathogen that produces a heat-stable enterotoxin (NAG-ST), is encapsulated. Here, we report the covalent structure and studies of the biogenesis of the capsule in V. cholerae NRT36S. Results. The structure of the capsular (CPS) polysaccharide was determined by high resolution NMR spectroscopy and shown to be a complex structure with four residues in the repeating subunit. The gene cluster of capsule biogenesis was identified by transposon mutagenesis combined with whole genome sequencing data (GenBank accession DQ915177). The capsule gene cluster shared the same genetic locus as that of the O-antigen of lipopolysaccharide (LPS) biogenesis gene cluster. Other than V. cholerae O139, this is the first V. cholerae CPS for which a structure has been fully elucidated and the genetic locus responsible for biosynthesis identified. Conclusion. The co-location of CPS and LPS biosynthesis genes was unexpected, and would provide a mechanism for simultaneous emergence of new O and K antigens in a single strain. This, in turn, may be a key element for V. cholerae to evolve new strains that can escape immunologic detection by host populations.

Original languageEnglish (US)
Article number20
JournalBMC microbiology
Volume7
DOIs
StatePublished - Apr 13 2007

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Vibrio cholerae non-O1
Vibrio cholerae
Capsules
Polysaccharides
Lipopolysaccharides
Multigene Family
Genes
O Antigens
Genetic Loci
Vibrio cholerae O139
Nucleic Acid Databases
Mutagenesis
Magnetic Resonance Spectroscopy
Genome

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S : Genes are embedded in the LPS region. / Chen, Yuansha; Bystricky, Peter; Adeyeye, Jacob; Panigrahi, Pinaki; Ali, Afsar; Johnson, Judith A.; Bush, C. A.; Morris, J. G.; Stine, O. C.

In: BMC microbiology, Vol. 7, 20, 13.04.2007.

Research output: Contribution to journalArticle

Chen, Y, Bystricky, P, Adeyeye, J, Panigrahi, P, Ali, A, Johnson, JA, Bush, CA, Morris, JG & Stine, OC 2007, 'The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: Genes are embedded in the LPS region', BMC microbiology, vol. 7, 20. https://doi.org/10.1186/1471-2180-7-20
Chen, Yuansha ; Bystricky, Peter ; Adeyeye, Jacob ; Panigrahi, Pinaki ; Ali, Afsar ; Johnson, Judith A. ; Bush, C. A. ; Morris, J. G. ; Stine, O. C. / The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S : Genes are embedded in the LPS region. In: BMC microbiology. 2007 ; Vol. 7.
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