The capsule and O antigen in Vibrio cholerae O139 Bengal are associated with a genetic region not present in Vibrio cholerae O1

L. E. Comstock, D. Maneval, P. Panigrahi, A. Joseph, M. M. Levine, J. B. Kaper, J. G. Morris, J. A. Johnson

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Vibrio cholerae O139 Bengal, although closely related to V. cholerae O1 El Tor, produces a polysaccharide capsule and has a distinct O antigen. We have identified a chromosomal region of at least 11 kb, as defined by three TnphoA mutations, that is required for the expression of both polysaccharides. Electron microscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis show that these TnphoA mutants have lost the abilities both to express capsule and to produce lipopolysaccharide beyond the core oligosaccharide. Reactivity with O139 typing serum and resistance to serum are also lost in the mutants. DNA probes for this region do not hybridize with O1 V. cholerae but do react with other vibrios, implying that the region was recently acquired.

Original languageEnglish (US)
Pages (from-to)317-323
Number of pages7
JournalInfection and immunity
Volume63
Issue number1
StatePublished - Jan 1 1995

Fingerprint

Vibrio cholerae O139
Vibrio cholerae O1
O Antigens
Capsules
Polysaccharides
Vibrio
DNA Probes
Serum
Oligosaccharides
Sodium Dodecyl Sulfate
Lipopolysaccharides
Polyacrylamide Gel Electrophoresis
Electron Microscopy
Mutation

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Comstock, L. E., Maneval, D., Panigrahi, P., Joseph, A., Levine, M. M., Kaper, J. B., ... Johnson, J. A. (1995). The capsule and O antigen in Vibrio cholerae O139 Bengal are associated with a genetic region not present in Vibrio cholerae O1. Infection and immunity, 63(1), 317-323.

The capsule and O antigen in Vibrio cholerae O139 Bengal are associated with a genetic region not present in Vibrio cholerae O1. / Comstock, L. E.; Maneval, D.; Panigrahi, P.; Joseph, A.; Levine, M. M.; Kaper, J. B.; Morris, J. G.; Johnson, J. A.

In: Infection and immunity, Vol. 63, No. 1, 01.01.1995, p. 317-323.

Research output: Contribution to journalArticle

Comstock, LE, Maneval, D, Panigrahi, P, Joseph, A, Levine, MM, Kaper, JB, Morris, JG & Johnson, JA 1995, 'The capsule and O antigen in Vibrio cholerae O139 Bengal are associated with a genetic region not present in Vibrio cholerae O1', Infection and immunity, vol. 63, no. 1, pp. 317-323.
Comstock LE, Maneval D, Panigrahi P, Joseph A, Levine MM, Kaper JB et al. The capsule and O antigen in Vibrio cholerae O139 Bengal are associated with a genetic region not present in Vibrio cholerae O1. Infection and immunity. 1995 Jan 1;63(1):317-323.
Comstock, L. E. ; Maneval, D. ; Panigrahi, P. ; Joseph, A. ; Levine, M. M. ; Kaper, J. B. ; Morris, J. G. ; Johnson, J. A. / The capsule and O antigen in Vibrio cholerae O139 Bengal are associated with a genetic region not present in Vibrio cholerae O1. In: Infection and immunity. 1995 ; Vol. 63, No. 1. pp. 317-323.
@article{23f08f66b7984e0594ac78a0e11f2f6e,
title = "The capsule and O antigen in Vibrio cholerae O139 Bengal are associated with a genetic region not present in Vibrio cholerae O1",
abstract = "Vibrio cholerae O139 Bengal, although closely related to V. cholerae O1 El Tor, produces a polysaccharide capsule and has a distinct O antigen. We have identified a chromosomal region of at least 11 kb, as defined by three TnphoA mutations, that is required for the expression of both polysaccharides. Electron microscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis show that these TnphoA mutants have lost the abilities both to express capsule and to produce lipopolysaccharide beyond the core oligosaccharide. Reactivity with O139 typing serum and resistance to serum are also lost in the mutants. DNA probes for this region do not hybridize with O1 V. cholerae but do react with other vibrios, implying that the region was recently acquired.",
author = "Comstock, {L. E.} and D. Maneval and P. Panigrahi and A. Joseph and Levine, {M. M.} and Kaper, {J. B.} and Morris, {J. G.} and Johnson, {J. A.}",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "63",
pages = "317--323",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - The capsule and O antigen in Vibrio cholerae O139 Bengal are associated with a genetic region not present in Vibrio cholerae O1

AU - Comstock, L. E.

AU - Maneval, D.

AU - Panigrahi, P.

AU - Joseph, A.

AU - Levine, M. M.

AU - Kaper, J. B.

AU - Morris, J. G.

AU - Johnson, J. A.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Vibrio cholerae O139 Bengal, although closely related to V. cholerae O1 El Tor, produces a polysaccharide capsule and has a distinct O antigen. We have identified a chromosomal region of at least 11 kb, as defined by three TnphoA mutations, that is required for the expression of both polysaccharides. Electron microscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis show that these TnphoA mutants have lost the abilities both to express capsule and to produce lipopolysaccharide beyond the core oligosaccharide. Reactivity with O139 typing serum and resistance to serum are also lost in the mutants. DNA probes for this region do not hybridize with O1 V. cholerae but do react with other vibrios, implying that the region was recently acquired.

AB - Vibrio cholerae O139 Bengal, although closely related to V. cholerae O1 El Tor, produces a polysaccharide capsule and has a distinct O antigen. We have identified a chromosomal region of at least 11 kb, as defined by three TnphoA mutations, that is required for the expression of both polysaccharides. Electron microscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis show that these TnphoA mutants have lost the abilities both to express capsule and to produce lipopolysaccharide beyond the core oligosaccharide. Reactivity with O139 typing serum and resistance to serum are also lost in the mutants. DNA probes for this region do not hybridize with O1 V. cholerae but do react with other vibrios, implying that the region was recently acquired.

UR - http://www.scopus.com/inward/record.url?scp=0028831296&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028831296&partnerID=8YFLogxK

M3 - Article

C2 - 7528734

AN - SCOPUS:0028831296

VL - 63

SP - 317

EP - 323

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 1

ER -