The capsid protein of Turnip Crinkle Virus overcomes two separate defense barriers to facilitate systemic movement of the virus in Arabidopsis

Mingxia Cao, Xiaohong Ye, Kristen Willie, Junyan Lin, Xiuchun Zhang, Margaret G. Redinbaugh, Anne E. Simon, Thomas J Morris, Feng Qu

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The capsid protein (CP) of Turnip crinkle virus (TCV) is a multifunctional protein needed for virus assembly, suppression of RNA silencing-based antiviral defense, and long-distance movement in infected plants. In this report, we have examined genetic requirements for the different functions of TCV CP and evaluated the interdependence of these functions. A series of TCV mutants containing alterations in the CP coding region were generated. These alterations range from single-amino-acid substitutions and domain truncations to knockouts of CP translation. The latter category also contained two constructs in which the CP coding region was replaced by either the cDNA of a silencing suppressor of a different virus or that of green fluorescent protein. These mutants were used to infect Arabidopsis plants with diminished antiviral silencing capability (dcl2 dcl3 dcl4 plants). There was a strong correlation between the ability of mutants to reach systemic leaves and the silencing suppressor activity of mutant CP. Virus particles were not essential for entry of the viral genome into vascular bundles in the inoculated leaves in the absence of antiviral silencing. However, virus particles were necessary for egress of the viral genome from the vasculature of systemic leaves. Our experiments demonstrate that TCV CP not only allows the viral genome to access the systemic movement channel through silencing suppression but also ensures its smooth egress by way of assembled virus particles. These results illustrate that efficient long-distance movement of TCV requires both functions afforded by the CP.

Original languageEnglish (US)
Pages (from-to)7793-7802
Number of pages10
JournalJournal of virology
Volume84
Issue number15
DOIs
StatePublished - Aug 1 2010

Fingerprint

Carmovirus
Turnip crinkle virus
Capsid Proteins
coat proteins
Arabidopsis
Viruses
viruses
Viral Genome
virion
Virion
Antiviral Agents
mutants
Open Reading Frames
genome
open reading frames
Virus Assembly
leaves
Protein Biosynthesis
vascular bundles
amino acid substitution

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

The capsid protein of Turnip Crinkle Virus overcomes two separate defense barriers to facilitate systemic movement of the virus in Arabidopsis. / Cao, Mingxia; Ye, Xiaohong; Willie, Kristen; Lin, Junyan; Zhang, Xiuchun; Redinbaugh, Margaret G.; Simon, Anne E.; Morris, Thomas J; Qu, Feng.

In: Journal of virology, Vol. 84, No. 15, 01.08.2010, p. 7793-7802.

Research output: Contribution to journalArticle

Cao, M, Ye, X, Willie, K, Lin, J, Zhang, X, Redinbaugh, MG, Simon, AE, Morris, TJ & Qu, F 2010, 'The capsid protein of Turnip Crinkle Virus overcomes two separate defense barriers to facilitate systemic movement of the virus in Arabidopsis', Journal of virology, vol. 84, no. 15, pp. 7793-7802. https://doi.org/10.1128/JVI.02643-09
Cao, Mingxia ; Ye, Xiaohong ; Willie, Kristen ; Lin, Junyan ; Zhang, Xiuchun ; Redinbaugh, Margaret G. ; Simon, Anne E. ; Morris, Thomas J ; Qu, Feng. / The capsid protein of Turnip Crinkle Virus overcomes two separate defense barriers to facilitate systemic movement of the virus in Arabidopsis. In: Journal of virology. 2010 ; Vol. 84, No. 15. pp. 7793-7802.
@article{e8135c65cb934360ad1052021c7f8033,
title = "The capsid protein of Turnip Crinkle Virus overcomes two separate defense barriers to facilitate systemic movement of the virus in Arabidopsis",
abstract = "The capsid protein (CP) of Turnip crinkle virus (TCV) is a multifunctional protein needed for virus assembly, suppression of RNA silencing-based antiviral defense, and long-distance movement in infected plants. In this report, we have examined genetic requirements for the different functions of TCV CP and evaluated the interdependence of these functions. A series of TCV mutants containing alterations in the CP coding region were generated. These alterations range from single-amino-acid substitutions and domain truncations to knockouts of CP translation. The latter category also contained two constructs in which the CP coding region was replaced by either the cDNA of a silencing suppressor of a different virus or that of green fluorescent protein. These mutants were used to infect Arabidopsis plants with diminished antiviral silencing capability (dcl2 dcl3 dcl4 plants). There was a strong correlation between the ability of mutants to reach systemic leaves and the silencing suppressor activity of mutant CP. Virus particles were not essential for entry of the viral genome into vascular bundles in the inoculated leaves in the absence of antiviral silencing. However, virus particles were necessary for egress of the viral genome from the vasculature of systemic leaves. Our experiments demonstrate that TCV CP not only allows the viral genome to access the systemic movement channel through silencing suppression but also ensures its smooth egress by way of assembled virus particles. These results illustrate that efficient long-distance movement of TCV requires both functions afforded by the CP.",
author = "Mingxia Cao and Xiaohong Ye and Kristen Willie and Junyan Lin and Xiuchun Zhang and Redinbaugh, {Margaret G.} and Simon, {Anne E.} and Morris, {Thomas J} and Feng Qu",
year = "2010",
month = "8",
day = "1",
doi = "10.1128/JVI.02643-09",
language = "English (US)",
volume = "84",
pages = "7793--7802",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "15",

}

TY - JOUR

T1 - The capsid protein of Turnip Crinkle Virus overcomes two separate defense barriers to facilitate systemic movement of the virus in Arabidopsis

AU - Cao, Mingxia

AU - Ye, Xiaohong

AU - Willie, Kristen

AU - Lin, Junyan

AU - Zhang, Xiuchun

AU - Redinbaugh, Margaret G.

AU - Simon, Anne E.

AU - Morris, Thomas J

AU - Qu, Feng

PY - 2010/8/1

Y1 - 2010/8/1

N2 - The capsid protein (CP) of Turnip crinkle virus (TCV) is a multifunctional protein needed for virus assembly, suppression of RNA silencing-based antiviral defense, and long-distance movement in infected plants. In this report, we have examined genetic requirements for the different functions of TCV CP and evaluated the interdependence of these functions. A series of TCV mutants containing alterations in the CP coding region were generated. These alterations range from single-amino-acid substitutions and domain truncations to knockouts of CP translation. The latter category also contained two constructs in which the CP coding region was replaced by either the cDNA of a silencing suppressor of a different virus or that of green fluorescent protein. These mutants were used to infect Arabidopsis plants with diminished antiviral silencing capability (dcl2 dcl3 dcl4 plants). There was a strong correlation between the ability of mutants to reach systemic leaves and the silencing suppressor activity of mutant CP. Virus particles were not essential for entry of the viral genome into vascular bundles in the inoculated leaves in the absence of antiviral silencing. However, virus particles were necessary for egress of the viral genome from the vasculature of systemic leaves. Our experiments demonstrate that TCV CP not only allows the viral genome to access the systemic movement channel through silencing suppression but also ensures its smooth egress by way of assembled virus particles. These results illustrate that efficient long-distance movement of TCV requires both functions afforded by the CP.

AB - The capsid protein (CP) of Turnip crinkle virus (TCV) is a multifunctional protein needed for virus assembly, suppression of RNA silencing-based antiviral defense, and long-distance movement in infected plants. In this report, we have examined genetic requirements for the different functions of TCV CP and evaluated the interdependence of these functions. A series of TCV mutants containing alterations in the CP coding region were generated. These alterations range from single-amino-acid substitutions and domain truncations to knockouts of CP translation. The latter category also contained two constructs in which the CP coding region was replaced by either the cDNA of a silencing suppressor of a different virus or that of green fluorescent protein. These mutants were used to infect Arabidopsis plants with diminished antiviral silencing capability (dcl2 dcl3 dcl4 plants). There was a strong correlation between the ability of mutants to reach systemic leaves and the silencing suppressor activity of mutant CP. Virus particles were not essential for entry of the viral genome into vascular bundles in the inoculated leaves in the absence of antiviral silencing. However, virus particles were necessary for egress of the viral genome from the vasculature of systemic leaves. Our experiments demonstrate that TCV CP not only allows the viral genome to access the systemic movement channel through silencing suppression but also ensures its smooth egress by way of assembled virus particles. These results illustrate that efficient long-distance movement of TCV requires both functions afforded by the CP.

UR - http://www.scopus.com/inward/record.url?scp=77954480699&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954480699&partnerID=8YFLogxK

U2 - 10.1128/JVI.02643-09

DO - 10.1128/JVI.02643-09

M3 - Article

VL - 84

SP - 7793

EP - 7802

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 15

ER -