The C5 variant of the butyrylcholinesterase tetramer includes a noncovalently bound 60 kDa lamellipodin fragment

Lawrence M Schopfer, Hervé Delacour, Patrick Masson, Jacqueline Leroy, Eric Krejci, Oksana Lockridge

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Humans with the C5 genetic variant of butyrylcholinesterase (BChE) have 30–200% higher plasma BChE activity, low body weight, and shorter duration of action of the muscle relaxant succinylcholine. The C5 variant has an extra, slow-moving band of BChE activity on native polyacrylamide gel electrophoresis. This band is about 60 kDa larger than wild-type BChE. Umbilical cord BChE in 100% of newborn babies has a C5-like band. Our goal was to identify the unknown, 60 kDa protein in C5. Both wild-type and C5 BChE are under the genetic control of two independent loci, the BCHE gene on Chr 3q26.1 and the RAPH1 (lamellipodin) gene on Chr 2q33. Wild-type BChE tetramers are assembled around a 3 kDa polyproline peptide from lamellipodin. Western blot of boiled C5 and cord BChE showed a positive response with an antibody to the C-terminus of lamellipodin. The C-terminal exon of lamellipodin is about 60 kDa including an N-terminal polyproline. We propose that the unknown protein in C5 and cord BChE is encoded by the last exon of the RAPH1 gene. In 90% of the population, the 60 kDa fragment is shortened to 3 kDa during maturation to adulthood, leaving only 10% of adults with C5 BChE.

Original languageEnglish (US)
Article number1083
JournalMolecules
Volume22
Issue number7
DOIs
StatePublished - Jul 1 2017

Fingerprint

Butyrylcholinesterase
genes
fragments
muscle relaxants
proteins
body weight
electrophoresis
loci
antibodies
peptides
gels
Genes
Exons
Native Polyacrylamide Gel Electrophoresis
Succinylcholine
Umbilical Cord
Electrophoresis
Antibody Formation
Muscle
Proteins

Keywords

  • Butyrylcholinesterase
  • C5 phenotype
  • Lamellipodin
  • RAPH1
  • Tetramer

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

The C5 variant of the butyrylcholinesterase tetramer includes a noncovalently bound 60 kDa lamellipodin fragment. / Schopfer, Lawrence M; Delacour, Hervé; Masson, Patrick; Leroy, Jacqueline; Krejci, Eric; Lockridge, Oksana.

In: Molecules, Vol. 22, No. 7, 1083, 01.07.2017.

Research output: Contribution to journalArticle

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abstract = "Humans with the C5 genetic variant of butyrylcholinesterase (BChE) have 30–200{\%} higher plasma BChE activity, low body weight, and shorter duration of action of the muscle relaxant succinylcholine. The C5 variant has an extra, slow-moving band of BChE activity on native polyacrylamide gel electrophoresis. This band is about 60 kDa larger than wild-type BChE. Umbilical cord BChE in 100{\%} of newborn babies has a C5-like band. Our goal was to identify the unknown, 60 kDa protein in C5. Both wild-type and C5 BChE are under the genetic control of two independent loci, the BCHE gene on Chr 3q26.1 and the RAPH1 (lamellipodin) gene on Chr 2q33. Wild-type BChE tetramers are assembled around a 3 kDa polyproline peptide from lamellipodin. Western blot of boiled C5 and cord BChE showed a positive response with an antibody to the C-terminus of lamellipodin. The C-terminal exon of lamellipodin is about 60 kDa including an N-terminal polyproline. We propose that the unknown protein in C5 and cord BChE is encoded by the last exon of the RAPH1 gene. In 90{\%} of the population, the 60 kDa fragment is shortened to 3 kDa during maturation to adulthood, leaving only 10{\%} of adults with C5 BChE.",
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