The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice

Shaohong Qu, Guifu Liu, Bo Zhou, Maria Bellizzi, Lirong Zeng, Liangying Dai, Bin Han, Guo Liang Wang

Research output: Contribution to journalArticle

341 Citations (Scopus)

Abstract

The broad-spectrum rice blast resistance gene Pi9 was cloned using a map-based cloning strategy. Sequencing of a 76-kb bacterial artificial chromosome (BAC) contig spanning the Pi9 locus led to identification of six tandemly arranged resistance-like genes with a nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) (Nbs1-Pi9-Nbs6-Pi9). Analysis of selected Pi9 deletion mutants and transformation of a 45-kb fragment from the BAC contig into the susceptible rice cultivar TP309 narrowed down Pi9 to the candidate genes Nbs2-Pi9 and Nbs3-Pi9. Disease evaluation of the transgenic lines carrying the individual candidate genes confirmed that Nbs2-Pi9 is the Pi9 gene. Sequence comparison analysis revealed that the six paralogs at the Pi9 locus belong to four classes and gene duplication might be one of the major evolutionary forces contributing to the formation of the NBS-LRR gene cluster. Semiquantitative reverse transcriptase (RT)-PCR analysis showed that Pi9 was constitutively expressed in the Pi9-resistant plants and was not induced by blast infection. The cloned Pi9 gene provides a starting point to elucidate the molecular basis of the broadspectrum disease resistance and the evolutionary mechanisms of blast resistance gene clusters in rice.

Original languageEnglish (US)
Pages (from-to)1901-1914
Number of pages14
JournalGenetics
Volume172
Issue number3
DOIs
StatePublished - Mar 1 2006

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Multigene Family
Nucleotides
Binding Sites
Genes
Bacterial Artificial Chromosomes
Leucine
Disease Resistance
Gene Duplication
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis
leucine-rich repeat proteins
Oryza
Organism Cloning
Infection

ASJC Scopus subject areas

  • Genetics

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The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. / Qu, Shaohong; Liu, Guifu; Zhou, Bo; Bellizzi, Maria; Zeng, Lirong; Dai, Liangying; Han, Bin; Wang, Guo Liang.

In: Genetics, Vol. 172, No. 3, 01.03.2006, p. 1901-1914.

Research output: Contribution to journalArticle

Qu, Shaohong ; Liu, Guifu ; Zhou, Bo ; Bellizzi, Maria ; Zeng, Lirong ; Dai, Liangying ; Han, Bin ; Wang, Guo Liang. / The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. In: Genetics. 2006 ; Vol. 172, No. 3. pp. 1901-1914.
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