The bovine herpesvirus type 1 envelope protein Us9 acidic domain is crucial for anterograde axonal transport

S. I. Chowdhury, M. C S Brum, C. Coats, Alan R Doster, Huiyong Wei, C. Jones

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, we examined the functional role of bovine herpesvirus type 1 (BHV-1) Us9 acidic domain residues 83-90 in the anterograde axonal transport of the virus in calves (natural host), rabbits, and in cultured neurons. A mutant virus strain lacking Us9 residues 83-90 (BHV-1 Us9 Δ83-90) and the rescued virus (BHV-1 Us9 R83-90) replicated efficiently in the nasal and ocular epithelium during primary infection and established latency in the trigeminal ganglia (TG). However, upon reactivation from latency, only the BHV-1 Us9 R83-90 virus was detected in nasal and ocular swabs of animals. In compartmentalized, rabbit primary dorsal root ganglia (DRG) neuron cultures, the Us9-deleted BHV-1, BHV-1 Us9 Δ83-90 and BHV-1 Us9 R83-90 viruses were transported efficiently in the retrograde direction. However, only the BHV-1 Us9 R83-90 virus was transported in an anterograde direction. These studies suggested that the Us9 acidic domain residues located between 83 and 90 were required for axonal anterograde transport.

Original languageEnglish (US)
Pages (from-to)270-279
Number of pages10
JournalVeterinary Microbiology
Volume152
Issue number3-4
DOIs
StatePublished - Sep 28 2011

Fingerprint

Bovine Herpesvirus 1
Axonal Transport
cattle
viruses
Viruses
proteins
eyes
neurons
rabbits
Rabbits
Bovine herpesvirus 1
Neurons
Trigeminal Ganglion
bovine herpesvirus 1 US9 protein
axonal transport
Nasal Mucosa
Spinal Ganglia
Nose
epithelium
calves

Keywords

  • Anterograde neuronal transport
  • BHV-1 envelope protein Us9
  • Compartmentalized primary neuronal cultures
  • Latency and reactivation
  • Trigeminal ganglia

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

The bovine herpesvirus type 1 envelope protein Us9 acidic domain is crucial for anterograde axonal transport. / Chowdhury, S. I.; Brum, M. C S; Coats, C.; Doster, Alan R; Wei, Huiyong; Jones, C.

In: Veterinary Microbiology, Vol. 152, No. 3-4, 28.09.2011, p. 270-279.

Research output: Contribution to journalArticle

Chowdhury, S. I. ; Brum, M. C S ; Coats, C. ; Doster, Alan R ; Wei, Huiyong ; Jones, C. / The bovine herpesvirus type 1 envelope protein Us9 acidic domain is crucial for anterograde axonal transport. In: Veterinary Microbiology. 2011 ; Vol. 152, No. 3-4. pp. 270-279.
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