The biological activity of cationic liposomes in drug delivery and toxicity test in animal models

Panpan Qi, Mei Cao, Liju Song, Chong Chen, Mingdong Liu, Ningzhe Li, Daoyan Wu, Jingshan Peng, Guoku Hu, Jian Zhao

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

In the study we made use of DOTAP (1,2-dioleoyl-3-trimethylammonium), DOPE (1,2-dioleoyl-snglycero-3-phosphoethanolamine) and PEG-PE (polyethylene glycol- polyethylene) to make cationic PEG-liposomes by ultrasonic dispersion method. The plasmid pGPU6 combined with cationic PEG-liposomes or Liopofectamin 2000 was used to transfect PC3 cells to judge the transfection efficiency. HE staining showed that the pGUP6-shAurora B plasmid/liposomes complex could significantly inhibit tumor growth in mice tumor model. The results indicated that there was no remarkable difference between the homemade liposomes and Lipofectamin 2000 after transfection, with transfection efficiency over 80%. And the homemade liposomes also had high transfection efficiency in vivo. No significant side effects were observed on weight, coat condition, behavior or appetite and the life span of mice treated with pGPU6-shAurora B were extended. Beyond that, there were no differences in mortality or in pathological changes to the heart, liver, spleen, lungs and kidneys among all the mice.

Original languageEnglish (US)
Pages (from-to)159-164
Number of pages6
JournalEnvironmental Toxicology and Pharmacology
Volume47
DOIs
Publication statusPublished - Oct 1 2016

    Fingerprint

Keywords

  • Cationic liposomes
  • DOPE
  • DOTAP
  • PEG-PE
  • Toxicity assessment

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

Cite this