The bacterial effector AvrPto targets the regulatory coreceptor SOBIR1 and suppresses defense signaling mediated by the receptor-like protein Cf-4

Jinbin Wu, Aranka M. Van Der Burgh, Guozhi Bi, Lisha Zhang, James R. Alfano, Gregory B. Martin, Matthieu H.A.J. Joosten

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Receptor-like proteins (RLPs) and receptor-like kinases (RLKs) are cell-surface receptors that are essential for detecting invading pathogens and subsequent activation of plant defense responses. RLPs lack a cytoplasmic kinase domain to trigger downstream signaling leading to host resistance. The RLK SOBIR1 constitutively interacts with the tomato RLP Cf-4, thereby providing Cf-4 with a kinase domain. SOBIR1 is required for Cf-4-mediated resistance to strains of the fungal tomato pathogen Cladosporium fulvum that secrete the effector Avr4. Upon perception of this effector by the Cf-4/SOBIR1 complex, the central regulatory RLK SOMATIC EMBRYOGENESIS RECEPTOR KINASE 3a (SERK3a) is recruited to the complex and defense signaling is triggered. SOBIR1 is also required for RLP-mediated resistance to bacterial, fungal ,and oomycete pathogens, and we hypothesized that SOBIR1 is targeted by effectors of such pathogens to suppress host defense responses. In this study, we show that Pseudomonas syringae pv. tomato DC3000 effector AvrPto interacts with Arabidopsis SOBIR1 and its orthologs of tomato and Nicotiana benthamiana, independent of SOBIR1 kinase activity. Interestingly, AvrPto suppresses Arabidopsis SOBIR1-induced cell death in N. benthamiana. Furthermore, AvrPto compromises Avr4-triggered cell death in Cf-4-transgenic N. benthamiana, without affecting Cf-4/SOBIR1/SERK3a complex formation. Our study shows that the RLP coreceptor SOBIR1 is targeted by a bacterial effector, which results in compromised defense responses.

Original languageEnglish (US)
Pages (from-to)75-85
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume31
Issue number1
DOIs
StatePublished - Jan 2018

Fingerprint

Phosphotransferases
receptors
phosphotransferases (kinases)
Lycopersicon esculentum
Proteins
proteins
Nicotiana benthamiana
Arabidopsis
Embryonic Development
pathogens
Cell Death
tomatoes
somatic embryogenesis
Oomycetes
Pseudomonas syringae
Cladosporium
cell death
Cell Surface Receptors
Passalora fulva
Pseudomonas syringae pv. tomato

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

The bacterial effector AvrPto targets the regulatory coreceptor SOBIR1 and suppresses defense signaling mediated by the receptor-like protein Cf-4. / Wu, Jinbin; Van Der Burgh, Aranka M.; Bi, Guozhi; Zhang, Lisha; Alfano, James R.; Martin, Gregory B.; Joosten, Matthieu H.A.J.

In: Molecular Plant-Microbe Interactions, Vol. 31, No. 1, 01.2018, p. 75-85.

Research output: Contribution to journalArticle

Wu, Jinbin ; Van Der Burgh, Aranka M. ; Bi, Guozhi ; Zhang, Lisha ; Alfano, James R. ; Martin, Gregory B. ; Joosten, Matthieu H.A.J. / The bacterial effector AvrPto targets the regulatory coreceptor SOBIR1 and suppresses defense signaling mediated by the receptor-like protein Cf-4. In: Molecular Plant-Microbe Interactions. 2018 ; Vol. 31, No. 1. pp. 75-85.
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abstract = "Receptor-like proteins (RLPs) and receptor-like kinases (RLKs) are cell-surface receptors that are essential for detecting invading pathogens and subsequent activation of plant defense responses. RLPs lack a cytoplasmic kinase domain to trigger downstream signaling leading to host resistance. The RLK SOBIR1 constitutively interacts with the tomato RLP Cf-4, thereby providing Cf-4 with a kinase domain. SOBIR1 is required for Cf-4-mediated resistance to strains of the fungal tomato pathogen Cladosporium fulvum that secrete the effector Avr4. Upon perception of this effector by the Cf-4/SOBIR1 complex, the central regulatory RLK SOMATIC EMBRYOGENESIS RECEPTOR KINASE 3a (SERK3a) is recruited to the complex and defense signaling is triggered. SOBIR1 is also required for RLP-mediated resistance to bacterial, fungal ,and oomycete pathogens, and we hypothesized that SOBIR1 is targeted by effectors of such pathogens to suppress host defense responses. In this study, we show that Pseudomonas syringae pv. tomato DC3000 effector AvrPto interacts with Arabidopsis SOBIR1 and its orthologs of tomato and Nicotiana benthamiana, independent of SOBIR1 kinase activity. Interestingly, AvrPto suppresses Arabidopsis SOBIR1-induced cell death in N. benthamiana. Furthermore, AvrPto compromises Avr4-triggered cell death in Cf-4-transgenic N. benthamiana, without affecting Cf-4/SOBIR1/SERK3a complex formation. Our study shows that the RLP coreceptor SOBIR1 is targeted by a bacterial effector, which results in compromised defense responses.",
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AU - Joosten, Matthieu H.A.J.

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